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二母颗粒中知母水提工艺优化
引用本文:卢君蓉,傅超美,陈秋薇,徐晓秋,胡慧玲. 二母颗粒中知母水提工艺优化[J]. 中国实验方剂学杂志, 2012, 18(12): 31-34
作者姓名:卢君蓉  傅超美  陈秋薇  徐晓秋  胡慧玲
作者单位:成都中医药大学药学院中药材标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137
基金项目:国家"十一五"科技支撑计划重点项目(2006BAI06A13)
摘    要:目的:优选二母颗粒中知母的水提工艺.方法:以HPLC测定知母皂苷BⅡ、芒果苷的含量及干膏收率为指标进行综合评价,采用正交试验法,考察溶媒用量、浸润时间、提取时间及提取次数等水提工艺参数.结果:提取次数对水提工艺有显著性影响,确定较佳提取工艺为A1B1C1D3,即知母饮片加6倍量水,不浸泡,提取3次,每次30 min.结论:该优选水提工艺稳定、合理可行.

关 键 词:二母颗粒  知母  知母皂苷BⅡ  芒果苷  正交设计
收稿时间:2012-02-01

Optimization of Water Extraction Process for Anemarrhena asphodeloides in Ermu Granule
LU Jun-rong,FU Chao-mei,CHEN Qiu-wei,XU Xiao-qiu and HU Hui-ling. Optimization of Water Extraction Process for Anemarrhena asphodeloides in Ermu Granule[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(12): 31-34
Authors:LU Jun-rong  FU Chao-mei  CHEN Qiu-wei  XU Xiao-qiu  HU Hui-ling
Affiliation:Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Provincial and State Constructed Key Laboratory Breeding Base of Systematic Research and Development of Chinese Herbal Medicine Resource, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137,China;Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Provincial and State Constructed Key Laboratory Breeding Base of Systematic Research and Development of Chinese Herbal Medicine Resource, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137,China;Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Provincial and State Constructed Key Laboratory Breeding Base of Systematic Research and Development of Chinese Herbal Medicine Resource, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137,China;Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Provincial and State Constructed Key Laboratory Breeding Base of Systematic Research and Development of Chinese Herbal Medicine Resource, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137,China;Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine, Provincial and State Constructed Key Laboratory Breeding Base of Systematic Research and Development of Chinese Herbal Medicine Resource, College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137,China
Abstract:Objective:To optimize water extraction process of Anemarrhena asphodeloides in Ermu granule.Method:With the content of timosaponin BⅡ,mangiferin and yield of dry extract as indexes to do comprehensive evaluation,orthogonal test was designed to investigate water extraction parameters,such as solvent usage,infiltration time,extraction time and extraction times.Result:Extraction times had significant effect on water extraction process,and optimum extraction process was A1B1C1D3,that A.asphodeloides pieces was added six times the amount of water without infiltration,extracted three times with 30 min each time.Conclusion:This optimized process was stable,reasonable and feasible.
Keywords:Ermu granule  Anemarrhena asphodeloides  timosaponin BⅡ  mangiferin  orthogonal test
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