舒心胶囊对缺氧诱导人脐静脉内皮细胞凋亡及相关基因表达的影响

观察舒心胶囊对缺氧诱导人脐静脉内皮细胞凋亡及相关基因表达的影响。体外培养人脐静脉内皮细胞 ,建立缺氧模型 ,以透射电子显微镜技术、流式细胞术 (膜联蛋白v/碘化丙啶 )双染色鉴定细胞凋亡 ;流式细胞术检测Fas、bcl 2及p5 3蛋白表达。缺氧培养 12h后 ,电镜显示细胞呈典型凋亡形态 ,双染色流式细胞仪检测凋亡细胞比例 (13.0 3%± 0 .96 % )显著高于正常组 (4 .4 7%± 0 .6 4 % ) (P <0 .0 1) ,而加入 5g/L舒心胶囊原液缺氧培养 12h后 ,凋亡细胞显著减少 (5 .0 3%± 0 .5 4 % ) (P <0 .0 1)。Fas蛋白表达在缺氧损伤过程中未见改变 ,舒心胶囊可使bcl 2蛋白表达升高 ,p5 3蛋白表达降低。舒心胶囊能够抑制缺氧诱导人脐静脉内皮细胞凋亡 ,可能与其升高bcl 2表达、降低p5 3表达有关。

Effect of Shuxin Capsule on Apoptosis Induced by Hypoxia and Apoptosis-related Genes Expression in Cultured Human Umbilical Vein Endothelial Cells

Aim To investigate the effects of Shuxin capsule on apoptosis induced by hypoxia and apoptosis-related genes expression in cultured human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured and hypoxic models were established. The presence of apoptosis was detected by electron microscope and flow cytometry assessment of Annexin v/PI stain. The protein levels of Fas, bcl-2 and p53 were examined by flow cytometry. Results After cultured 12 h in hypoxic condition, apoptosis of HUVECs was identified with electron microscope and showed by flow cytometry. Apoptosis rate in hypoxia group (13.03%±0.96%) was significantly higher than that in control group (4.47%±0.64%) (p<0.01), and after treated with 5 g/L Shuxin capsule liquid for 12 h in hypoxic condition, apoptosis rate decreased clearly (5.03%±0.54%) (p<0.01). Furthermore, it was revealed by flow cytometry that the apoptpsis process was associated with upregulation of p53 expression and downregulation of bcl-2 expression,but not with the marked change of Fas expression. Shuxin capsule could make expression of p53 down-regulated and expression of bcl-2 upregulated. Conclusion Shuxin capsule can inhibit the apoptosis of HUVECs induced by hypoxia,which is associated with its effects on expression of bcl-2 and p53.