肝再生增强因子在肝癌细胞中的表达及意义

目的研究肝再生增强因子(ALR)对肝癌细胞和原代大鼠肝细胞增殖作用的影响及在肝细胞癌(HCC)中的表达。方法将不同种属的ALR分别与原代大鼠肝细胞和人肝癌细胞株QGY和HepG2共同培养后，用^3H-胸腺嘧啶核苷掺入法测定这些细胞的增殖情况。并应用免疫组织化学法对9例正常肝组织和21例HCC组织中ALR的表达进行了研究。结果不同种属的ALR在体外均能刺激人肝癌细胞株QGY和HepG2增殖，并呈剂量依赖关系，但对原代大鼠肝细胞均无刺激增殖作用。在正常肝组织中ALR不表达，但在HCC肝组织中ALR均表达，且ALR的表达程度与HCC分化程度和大小均无关。结论ALR可能在HCC的发生发展中起着重要作用。

Expression of augmenter of liver regeneration in hepatic tumor cells and its clinical significance

Objective To investigate the effects of augmenter of liver regeneration (ALR) on the proliferation of hepatocytes and hepatic tumor cells and the expression of ALR in herpatocellular carcinoma (HCC). Methods Primary rat hepatocytes, QGY and HepG2 cells were cultured separately with ALR from different species. Cell proliferation was detected by their 3H-TdR uptake. The expression of ALR was examined in 9 normal hepatic tissues and 21 HCC cases using immunohistochemistry method. Results Different ALRs could stimulate the proliferation of HepG2 and QGY cells in a dose-dependent way in vitro, but all ALR had no influence in the proliferation of primary rat hepatocytes. The expression of ALR was absent in normal hepatic tissues, but present in all HCC hepatic tissues. However, the expression of ALR had no relationship with the differentiation and size of the carcinomas. Conclusion ALR might play an important role in the occurrence and development of HCC.