卡托普利对培养大鼠窦房结细胞缺血损伤保护的形态学研究

目的: 研究培养的大鼠窦房结(SAN)细胞缺血损伤的形态学变化及卡托普利对缺血损伤的保护作用。方法: 用新生4 d SD大鼠150只。(1)细胞培养和制备缺血损伤模型；(2)活细胞观察；(3)HE染色；(4)透射电镜实验。结果: 缺血60 min时培养细胞的活力及结构开始受损伤，90 min时损伤表现明显，120 min时表现为严重损伤。而卡托普利处理组，细胞受损伤程度明显减轻。结论: 随着缺血时间的延长，培养SAN细胞缺血性损伤渐加重，而卡托普利对该细胞有明显的保护作用。

The morphological study of protective effects of captopril on cultured SAN cells of newborn rats in ischemic model

Objective: To investigate the morphological changes of the cultured SAN cells in ischemia model and the protective effects of captopril on the SAN cells with ischemic injury. Methods: One hundred and fifty SD rats of 4 d old were used to develop primary cell culture of SAN cells. Then, living cells which included the cells in normal growing condition and in ischemic model were observed. In addition, living cells of SAN were fixed and stained with HE, and observed by light microscopy and transmission electromicroscopy(TEM). Results: The vitality, the morphologic characteristic and architecture of cultured SAN cells were slightly injured at 60 min,obviously injured at 90 min and seriously injured at 120 min following ischemia, whereas in the group treated with captopril the injuries were found to be significantly relieved. Conclusion:The ischemic injuries of cultured SAN cells in ischemia model were intensively developed with prolonged ischemia and the obvious protective effects of captopril on cultured SAN cells in ischemic model were observed.