豆腐果苷对骨关节炎大鼠软骨和滑膜组织影响

目的研究豆腐果苷对骨关节炎大鼠软骨结构和滑膜组织的影响。方法碘乙酸钠注射 SD雄性大鼠膝关节造模成功后，随机区组分组法分为三组，即假手术组、模型组、豆腐果苷 50 mg·kg.1·d.1组。豆腐果苷灌胃 21 d后，取大鼠膝关节制成组织切片， HE染色观察膝关节软骨和滑膜病理变化，并进行评分；番红固绿染色观察大鼠膝关节软骨病理变化，并进行 Mankin评分。研究起止时间为 2019年 12月至 2020年 7月。结果相对于假手术组，模型组大鼠膝关节软骨和滑膜均受到不同程度损伤，滑膜病理评分显著升高［( 6.88±0.83)分比( 0.50±0.53)分］(P<0.01)且软骨结构、软骨细胞和番红固绿染色的 Mankin评分也显著升高［分别是( 3.88±0.83)分比( 0.50±0.53)分，(1.75±0.46)分比(0，.25±0.46)分，(2.38±0.74)分比( 0.38±0.52)分］(P<0.01)。然而，连续给予豆腐果苷灌胃 21 d后，相对于模型组，大鼠的关节软骨结构和滑膜组织损伤程度均有显著改善，膝关滑膜病理评分显著降低［(3.50±0.53)分比(6.88±0.83)分］(P<0.01)且软骨结构、软骨细胞和番红固绿染色的 Mankin评分也显著降低［分别为( 1.63±0.52)分，(1.13±0.64)分，(0.88±0.64)分］(P<0.0，5)。结论豆腐果苷可减轻骨关节炎大鼠膝关节软

Effect of helicid on cartilage and synovium tissue of osteoarthritis rats

Objective To evaluate the effects of helicid on pathologic changes of cartilage and synovium in osteoarthritis (OA) rats. Methods After the knee joints of SD male rats were successfully molded by sodium iodoacetate injection, they were random blockgrouping assigned into three groups, namely, sham-operated group, model group, and helicid 50 mg·kg.1 ·d.1 group. After 21 days of he.licid gavage, tissue sections were taken from the knee joints of rats, and the pathological changes of knee cartilage and synovial mem.brane were observed by hematoxylin & eosin (HE) staining and scored; the pathological changes of knee cartilage of rats were observedby Safranin O-Fast Green staining and scored by Mankin. The study starts and ends from December 2019 to July 2020.Results Com. pared with the sham-operated group, the knee cartilage and synovium of the model group were damaged to different degrees, and the sy.novial pathologcal scores were significantly higher (6.88±0.83) vs. (0.50±0.53)] (P<0.01), and Mankin scores for cartilage structure, chondrocytes, and Safranin O-Fast Green staining were significantly higher (3.88±0.83) vs. (0.50 ±0.53), (1.75±0.46) vs. (0.25±0.46), and (2.38±0.74) vs. (0.38±0.52), respectively] (P<0.01). However, after 21 days of continuous administration of helicid gavage, com.pared with the model group, the degree of damage to articular cartilage structures and synovial tissues in the rats was significantly im.proved, with a significant decrease in the knee joint synovial pathological score (3.50±0.53) vs. (6.88±0.83)] (P<0.01) and a significant decrease in the Mankin scores for cartilage structure, chondrocytes and Safranin O-Fast Green staining (1.63±0.52), (1.13±0.64), and (0.88±0.64), respectively] (P<0.05).Conclusion Helicid could reduce the injury of cartilage structure and the synovial tissue in kneejoints of rats with osteoarthritis and has the effect of reducing synovial inflammation and protecting cartilage.