胎儿淋巴细胞在妊娠期肝内胆汁淤积症中的作用

目的：探讨胎儿淋巴细胞在ICP发病机制的可能作用。方法：采用单向混合淋巴细胞反20例ICP患者（研究组）及正常孕妇（对照组）的胎儿淋巴细胞对母体外周血的单个核细胞（PBM可溶性抗原的增殖情况。结果：（1）胎儿脐血中的淋巴细胞与母体已灭活的单个核细胞单向混合淋淋巴细胞的增殖程度显著高于正常组；（2）ICP患者胎儿脐血中的淋巴细胞对母体蜕膜组织的增殖高于正常组。结论：（1）胎儿淋巴细胞可能是ICP发病过程中主要的效应细胞之一。（2）ICP患者耐受失衡可能是ICP发病的重要机制之一。

The Fetal Lymphocyte on Pathogenesis of Intrahepatic Cholestasis of Pregnancy

Objective:To explore effect of the fetal lymphocyte on pathogenesis of intrahepatic cholestasis of pregnancy (ICP).Methods:20 pregnant women with ICP and 20 normal pregnancy women were enrolled in the study.The Singal Mixed Lymphocyte Culture/Reaction,(MLC/MLR) was conducted using inactive peripheral blood mononuclear (PBMC) obtained from maternal peripheral blood and cord blood from fetus.And Antigen-induced-lymphocyte-proliferation-reaction was used decidual solube antigen obtained from maternal peripheral blood and cord blood from fetus.The intense of proliferation was calculated and compared between normal and ICP-complicated pregnancies.Result:(1) The level of intense of proliferation of fetal lymphocyte was significantly increased in ICP group than those of normal control group in Singal Mixed Lymphocyte Culture.(2) The level of intense of proliferation of fetal lymphocyte was significantly increased in ICP group than those of normal control group in decidual soluble antigen induced lymphocyte proliferation reaction.Conclusions:(1) The fetal lymphocyte may be one of the effective cells on pathogenesis of ICP.(2)The disturbance of fatal-maternal immune-tolerance is one of the important mechanisms in ICP.