多巴胺和多巴酚丁胺对临床部分生化检测项目的干扰

目的评估多巴胺和多巴酚丁胺对基于过氧化物和过氧化物酶反应原理的6个生化检测项目肌酐(Cr)、尿酸(UA)、葡萄糖(Glu)、总胆固醇(TC)、甘油三酯(TG)、糖化血清蛋白(GSP)]的影响。方法配制含有100.00、50.002、5.00、12.506、.253、.131、.57μg/mL不同药物(多巴胺、多巴酚丁胺)浓度的血清,采用日立7600全自动生化仪(简称日立7600)测定血清Cr、UA、Glu、TC、TG、GSP浓度;采用强生VITROSR○5.1 FS干式生化仪(简称强生VITROS)检测Cr、UA和Glu浓度。计算不同血药浓度对各个项目干扰的程度。结果随着药物浓度逐渐增加,6个检测项目的检测结果均逐渐下降。血药浓度≤1.57μg/mL时对除GSP外的5个日立7600检测项目的负干扰<10%;血药浓度≥50μg/mL时对强生VITROS的Cr、UA测定结果的负干扰>10%。各个浓度的药物对强生VITROS检测Glu没有明显影响(<10%)。结论高浓度的多巴胺和多巴酚丁胺(≥1.57μg/mL)对日立7600的检测Cr、UA、Glu、TC、TG和GSP呈明显负干扰。强生VITROS抗多巴胺和多巴酚丁胺的干扰能力明显强于日立7600上的相应检测项目。

Dopamine and dobutamine interference in some biochemical assays

Objective To evaluate dopamine and dobutamine interference in 6 biochemical assays creatinine（Cr）,uric acid（UA）,glucose（Glu）,total cholesterol（TC）,triglyceride（TG） and glycated serum protein（GSP）] based on the peroxide and peroxidase reaction principle.Methods Sera containing different concentrations of dopamine or dobutamine（100.00,50.00,25.00,12.50,6.25,3.13 and 1.57 μg/mL） were prepared.Cr,UA,Glu,TC,TG and GSP were measured on a Hitachi 7600 automatic biochemical analyzer（Hitachi 7600）.Cr,UA and Glu were measured on a VITROS ○ R 5.1 FS dry type biochemical analyzer（VITROS）.The interference of each concentration drug was calculated.Results The measured 6 assays′ results decreased gradually with the increasing of the drug concentration.The negative interference of 5 assays（except GSP） measured on the Hitachi 7600 was 10% in the drug concentration ≤1.57μg/mL.The nagative interference of Cr and UA measured on the VITROS was 10% in the drug concentration ≥50 μg/mL.There was no significant interference（10%） of Glu measured on the VITROS in all the different drug concentrations.Conclusions Dopamine and dobutamine in the drug concentration ≥1.57 μg/mL have significant negative interference of biochemical assays（Cr,UA,Glu,TC,TG and GSP） on Hitachi 7600.The biochemical assays measured on the VITROS are less interfered by the drugs than those on the Hitachi 7600.