RP-HPLC同时测定槐角丸中4种有效成分的含量

 目的 建立同时测定槐角丸中槐角苷、黄芩苷、染料木素和汉黄芩素4种有效成分含量的HPLC方法。方法 采用Diamonsil C₁₈柱(4.6 mm×200 mm， 5 μm进行分离；流动相为乙腈-0.05%磷酸溶液，梯度洗脱(0～10 min，25%～37%乙腈；10～25 min，37% ～60% 乙腈；检测波长260 nm；柱温30 ℃；流速1.0 mL·min-1；进样量10 μL。结果 槐角苷、黄芩苷、染料木素和汉黄芩素的质量浓度与峰面积分别在11.6~116 μg·mL-1(r=0.999 6， n=6、33.5~335 μg·mL-1 (r=0.999 8， n=6、0.46~4.63 μg·mL-1 (r=0.999 8， n=6和1.54~15.4 μg·mL-1(r=0.999 7， n=6内呈良好的线性关系；平均加样回收率依次为99.3%、99.6%、99.6%和99.0%。RSD为1.1%、1.1%、1.4%和0.8%。结论 该方法快速、简便、重复性好，适合于同时测定槐角丸中槐角苷、黄芩苷、染料木素和汉黄芩素的含量。

Simultaneous Determination of Four Effective Components in Huaijiao Pills by RP-HPLC

OBJECTIVE To develop a RP-HPLC method for the determination of sophoricosid， baicalin， genistein and wogonin in Huaijiao pills. METHODS A Diamonsil C₁₈ column (4.6 mm×200 mm， 5μm was used at 30 ℃. The mobile phase consisted of acetonitrile-0.05% phosphoric acid aqueous under gradient elution(0-10 min， 25%-37% acetonitrile； 10-25 min， 37%-60% acetonitrile. The flow rate was set at 1.0 mL·min-1. The detection wavelength was 260 nm. RESULTS The linearities ranged in 11.6-116 μg·mL-1 for sophoricosid (r=0 999 6，n=6， 33.5-335 μg·mL-1 for baicalin (r=0.999 8， n=6， 0.46-4.63 μg·mL-1 for genistein (r=0.999 8， n=6， 1.54-15.4 μg·mL-1 for wogonin (r=0.999 7， n=6 respectively. Recoveries were 99.3% with RSD 1.1% for sophoricosid， 99.6% with RSD 1.1% for baicalin，99.6% with RSD 1.4% for genistein，99.0% with RSD 0.8% for wogonin. CONCLUSION The method is quick， simple and repeatable for simultaneous determination of sophoricosid， baicalin， genistein and wogonin in Huaijiao pills.