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Euprosterna elaeasa virus genome sequence and evolution of the Tetraviridae family: Emergence of bipartite genomes and conservation of the VPg signal with the dsRNA Birnaviridae family |
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| Authors: | Jean-Louis Zeddam Karl H.J. Gordon Cristiano A. Felipe Alves Terry N. Hanzlik Alexander E. Gorbalenya |
| Affiliation: | a IRD, UR 072, Laboratoire Evolution, Génomes et Spéciation, UPR 9034, CNRS, 91198 Gif-sur-Yvette Cedex, France b Université Paris-Sud 11, 91405 Orsay Cedex, France c CSIRO Entomology, GPO Box 1700, Canberra ACT 2601, Australia d Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Postzone E4-P, P.O. Box 9600, NL-2300 RC Leiden, The Netherlands e Department of Microbiology and Immunology, School of Medical Sciences, University of Otago, PO Box 56, Dunedin, 9054, New Zealand f Advanced Biomedical Computing Center, Science Applications International Corporation/National Cancer Institute, P.O. Box B, Frederick, MD 21702-1201, USA |
| Abstract: | The Tetraviridae is a family of non-enveloped positive-stranded RNA insect viruses that is defined by the T = 4 symmetry of virions. We report the complete Euprosterna elaeasa virus (EeV) genome sequence of 5698 nt with no poly(A) tail and two overlapping open reading frames, encoding the replicase and capsid precursor, with ∼67% amino acid identity to Thosea asigna virus (TaV). The N-terminally positioned 17 kDa protein is released from the capsid precursor by a NPGP motif. EeV has 40 nm non-enveloped isometric particles composed of 58 and 7 kDa proteins. The 3′-end of TaV/EeV is predicted to form a conserved pseudoknot. Replicases of TaV and EeV include a newly delineated VPg signal mediating the protein priming of RNA synthesis in dsRNA Birnaviridae. Results of rooted phylogenetic analysis of replicase and capsid proteins are presented to implicate recombination between monopartite tetraviruses, involving autonomization of a sgRNA, in the emergence of bipartite tetraviruses. They are also used to revise the Tetraviridae taxonomy. |
| Keywords: | Tetraviridae Birnaviridae Insect virus Genome sequence Permuted RNA-dependent RNA polymerase VPg Jelly-roll-based capsid Phylogeny Recombination Taxonomy |
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