促性腺激素释放激素类似物对大鼠中缝核簇5-HT样阳性物质表达的影响

研究促性腺激素释放激素类似物 ( Gn RH-A)对大鼠中缝核簇 5 -HT样阳性物质表达的影响 ,采用酶联免疫吸附试验测定血清激素水平 ,用免疫组织化学方法和图像分析技术观察大鼠中缝核簇 5 -HT样阳性物质的表达。结果证明 :( 1) Gn RH-A组大鼠血清 17β-雌二醇 ( E2 )、LH及 FSH水平较正常对照组下降 ,差异显著 ( P<0 .0 5 ) ;与去卵巢组 ( OVX)比较 ,E2 相似 ,差异不显著 ( P>0 .0 5 ) ,但 L H、F SH较低 ( P<0 .0 5 ) ;与 Gn RH-A+E2 组比较 E2 浓度较低 ,差异显著 ( P<0 .0 5 ) ,L H、FSH相似 ( P>0 .0 5 )。( 2 ) 5 -HT样阳性物质在大鼠中缝背核 ,中缝正中核表达较多 ,Gn RH-A组大鼠中缝背核 ,中缝正中核 5 -HT样阳性细胞数及光密度值都低于正常对照组 ( P<0 .0 5 ) ,与去卵巢组相似 ( P>0 .0 5 )。 ( 3 ) Gn RH-A、17β-雌二醇联合用药后 ,大鼠中缝背核 ,中缝正中核 5 -HT样阳性细胞数及光密度值都与正常对照组相似 ,无显著差异 ( P>0 .0 5 )。结论 :Gn RH-A可改变大鼠中缝背核 ,中缝正中核 5 -HT样阳性物质表达 ,从而影响其神经元的功能 ,此点可能与其副作用类似绝经综合征有关。

EFFECT OF GnRH-A ON CHANGES OF 5-HT LIKE IMMUNOREACTIVITIES IN RAPHEAL NUCLEI COMPLEX OF THE RAT

To investigate the effect of gonadotropin releasing hormone analogue(GnRH-A) on changes of 5-HT like immunoreactivities(5-HT LI) in rapheal nuclei complex of the rat, ELISA, immunohistochemistry and image analysis technique were used to measure serum hormone levels and to assay the expression of 5-HT LI respectively. The results demonstrated that: (1) compared with the normal group, concentrations of serum E 2, LH and FSH decreased in the GnRH-A group (P<0.05); while concentration of E 2 was similar to that of the OVX group(P>0.05), but concentrations of LH and FSH were significantly lower(P<0.05). The concentration of serum E 2 of GnRH-A+E 2 group was lower than that of the GnRH-A group(P<0.05). (2) 5-HT LI was expressed significantly in the nucleus raphe dorsalis and median nucleus of rats. GnRH-A caused a decline in cell numbers and optical density(OD) of 5-HT LI(P<0.05) in both sites, which were similar to the results of the OVX rats(P>0.05). (3) Adding E 2 could reverse the changes caused by GnRH-A, which were similar to the normal rats(P>0.05). The results indicate that GnRH-A can change the expression of 5-HT in the nucleus raphe dorsalis and median nucleus, and then change the function of neurons, which may be associated with its side effects, such as menopause symptom.