精神分裂症与载脂蛋白E基因的关联研究

目的 探讨汉族人群载脂蛋白E(ApoE)基因与精神分裂症的相关性.方法 应用聚合酶链反应扩增技术及限制性片段长度多态性测定100例精神分裂症患者(男女各50例)及64例正常对照(男女各32例)的ApoE基因型分布和等位基因频率.结果 ApoE基因型总体分布患者组(ε2/2,1%;ε2/3,6%;ε2/4,1%;ε3/3,78%;ε3/4,13%;ε4/4,1%)和对照组(ε2/2,0;ε2/3,13%;ε2/4,3%;ε3/3,64%;ε3/4,19%;ε4/4,2%)差异无显著性(P＞0.05),ApoE等位基因总体分布患者组(ε2,5%;ε3,88%;ε4,8%)和对照组(ε2,8%;ε3,80%;ε4,13%)差异无显著性(P＞0.05).女性患者组与女性对照组的ApoE基因型分布存在显著差异,女性病人的ε3/3基因型频率(RR=3.84,P＜0.05)和ε3等位基因型频率(RR=3.06,P＜0.05)明显高于女性对照组.结论 ApoE基因对精神分裂症易患性的影响可能存在性别上的差异,ε3等位基因可能与女性精神分裂症的发病相关联.

An association study of apolipoprotein E gene and Chinese schizophrenic patients

Objective To investigate the association between apolipoprotein E (ApoE) gene and schizophrenia in Han Chinese. Methods The polymorphism of ApoE gene in 100 schizophrenic in-patients (50 men and 50 women) and 64 normal controls (32 men and 32 women) were genotyped with polymerase chain reaction and restriction fragment length polymorphism techniques. Results There was no difference in the genotype distributions of patients(ε2/2,1%;ε2/3,6%;ε2/4,1%;ε3/3,78%;ε3/4,13%;ε4/4,1%) and controls(ε2/2,0;ε2/3,13%;ε2/4,3%;ε3/3,64%;ε3/4,19%;ε4/4,2%)(P＞0.05). There was no difference in the allele frequencies of patients(ε2,5%;ε3,88%;ε4,8%) and controls(ε2,8%;ε3,80%;ε4,13%)(P＞0.05). There was significant difference in the genotype distributions of female patients and female controls. Compared to controls, the female patients with schizophrenia had significantly increased ε3/3 genotype (RR=3.84,P＜0.05) and ε3 allele (RR=3.06,P＜0.05) . Conclusion The influence of the ApoE gene on the susceptibility of schizophrenia may be different between the genders of patients. Women with ε3 allele may have an increased risk of schizophrenia.