| pcDNA_(3.1)-OGP基因转染兔骨髓基质干细胞的表达及生物学效应的观察 |
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| 引用本文： | 安刚,吕松岑,邓秋奎,薛震,李冀宏.pcDNA_(3.1)-OGP基因转染兔骨髓基质干细胞的表达及生物学效应的观察[J].中国矫形外科杂志,2010,18(3):246-249. |
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| 作者姓名： | 安刚 吕松岑 邓秋奎 薛震 李冀宏 |
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| 作者单位： | 1. 哈尔滨医科大学附属第二医院骨科,哈尔滨,150086
2. 哈尔滨医科大学生化教研室,哈尔滨,150086 |
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| 基金项目： | 黑龙江省留学回国人员基金项目(编号:LC04C03) |
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| 摘 要： | 目的]观察成骨生长肽(osteogenic growth peptid,OGP)基因转染兔骨髓基质干细胞后的表达及表达产物对骨髓基质干细胞向成骨细胞分化的影响.方法]构建重组真核表达载体pcDNA_(3.1)-OGP,并在脂质体介导下,将其导入兔骨髓基质干细胞.通过G418筛选获得阳性克隆.用RT-PCR方法榆测OGP基因在骨髓基质干细胞内的表达.Ⅰ型胶原和碱性磷酸酶的检测观察转染pcDNA_(3.1)-OGP后骨髓基质干细胞向成骨细胞分化情况.结果]成功构建真核表达载体pcDNA_(3.1)-OGP,用RT-PCR方法及碱性磷酸酶和Ⅰ型胶原检测证实OGP基因能在骨髓基质干细胞中表达并促进其向成骨细胞分化.结论]经pcDNA_(3.1)-OGP转染的兔骨髓基质干细胞不仅可以表达OGP,而且具有向成骨细胞系分化的特性.
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| 关 键 词： | 成骨生长肽 peDNA_(3.1)载体 骨髓基质干细胞 基因转染 |
Expression and biological effects of pcDNA_(3.1)-OGP gene transfection on rabbit bone marrow stromal cells in vitro |
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| AN Gang,LV Song-cen,DENG Qiu-kui,et al..Expression and biological effects of pcDNA_(3.1)-OGP gene transfection on rabbit bone marrow stromal cells in vitro[J].The Orthopedic Journal of China,2010,18(3):246-249. |
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| Authors: | AN Gang LV Song-cen DENG Qiu-kui |
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| Institution: | AN Gang,LV Song-cen,DENG Qiu-kui,et al.The Second Hospital of Harbin Medical Universicy,Harbin 150086,China |
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| Abstract: | Objective]To observe the expression of osteogenic growth peptide(OGP) gene,which had been transfected into rabbit bone marrow stromal cells(BMSC) by adenovirus,and detect the proliferation and differentiation of rabbit bone marrow stromal cells after gene transfection in vitro.Methods]pcDNA3.1-OGP was constructed by using gene clone and recombined technique.With the help of lipofectamine 2000,BMSC were transfected with pcDNA3.1-OGP.The positive cell clones were selected with G418.The expression of OGP gen... |
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| Keywords: | ostoogenic growth peptied pcDNA3 1 vector bone marrow stromal cells gene transfection |
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