Substitutions at the Glu62 residue of human interleukin-2 differentially affect its binding to the α chain and the βγ complex of the interleukin-2 receptor

Human interleukin-2 (IL-2) α helix B is more conserved than the whole molecule, but has been less studied than other α helices of IL-2. Using site-directed mutagenesis, several IL-2 mutants in this helix were obtained. We found that the IL-2 mutant containing Leu at position 62 (Leu⁶²-IL-2) loses its ability to bind IL-2 receptor subunit α (IL-2Rα), but retains binding affinity to IL-2R subunit βγ as well as some bioactivity; nevertheless, another substitution at the same residue, Arg⁶² IL-2, loses its binding ability to both IL-2Rα and IL-2Rβγ, and can no longer stimulate IL-2-dependent cell growth, showing that Glu⁶² not only takes part in IL-2Rα binding, but can also affect IL-2 binding to IL-2Rβγ. In this regard, Glu⁶² may be a key site in the IL-2/IL-2Rα interaction, and can facilitate IL-2R ternary-complex formation, leading to IL-2Rα-mediated, IL-2-stimulated signal transduction.