苏木对C57BL/6小鼠Lewis肺癌不同时间生长和转移的影响

目的动态观察苏木对Lewis肺癌生长和转移的影响并研究其部分作用机制。方法建立Lewis肺癌C57BL/6小鼠肿瘤模型,随机分为模型组,苏木低、高剂量组,化疗组,化疗加苏木低、高剂量组共6组,连续灌胃给药21天。于第7、14、21天分3批处死小鼠,动态观察各组抑瘤率、抑转移率,流式细胞仪检测小鼠移植瘤细胞CD44的表达,酶联免疫吸附法(ELISA)检测小鼠血清P-选择素浓度。结果除苏木低剂量组外,其余各给药组在3个时间点瘤重均低于模型组(P0.05,P0.01);且化疗加苏木高剂量组低于同期化疗组(P0.05,P0.01)。瘤细胞CD44~+表达:第7天,苏木高剂量组、化疗组均显著低于模型组(P0.01);第14天,化疗加苏木低、高剂量组低于模型组(P0.01,P0.05);第21天,化疗加苏木低、高剂量组显著低于模型组(P0.01)。P-选择素浓度:第7天,各给药组低于模型组(P0.05,P0.01);第14天,用药各组与模型组比较差异无统计学意义(P0.05);第21天,化疗加苏木高剂量组低于模型组(P0.05)。结论苏木及苏木联合化疗可以抑制Lewis肺癌的生长,并可以抑制小鼠瘤细胞CD44、血清P-选择素等黏附因子的表达。其抑制转移的机制可能与降低CD44、P-选择素的表达有关。

Effects of Lignum Sappan on Growth and Metastases of Lewis Lung Carcinoma at Different Phases in C57BL/6 Mice

Objective To observe the effects of Lignum Sappan （LS） on the growth and metastases of transplanted Lewis lung carcinoma （LLC） in mice and investigate its partial mechanism of action. Methods C57BL/6 mice were established in LLC model and divided into six groups in random： Group A was untreated; Group B was treated by chemotherapy （CM） only; Groups C-F were treated respectively with low-dose LS, high-dose LS, CM ＋ low-dose LS and CM ＋ high-dose LS, via intragastric administration for 21 successive days. Mice were sacrificed in batches at different time points （ d 7, d 14 and d 21 ） to observe the tumor inhibition rate and the metastases suppressing rate was measured dynamically. Meantime, the CD44 expression in tumor cells was measured by flow cytometry and serum P-selectin concentration was measured by ELISA assay. Results Tumor weight in all treated groups, except Group C, was lower than that in Group A at the three time points （P〈0.05, P〈0.01 ）, and that was lower in Group F than in Group B at the corresponding time points （P〈0.05, P〈0.01）. Comparisons of CD44 ^＋ in tumor cells showed that as compared with Group A, on d 7, it was lower in Groups B and D （P〈0.01）; on d 14, it was lower in Group E （P〈0.01） and Group F （P〈 0.05） ; and on d 21, it was lower in Groups E and F （P 〈0.01 ）. As for the concentration of P-selectin, the intergroup comparisons showed that it was lower in Groups B-F on d 7 and in Group F on d 21 than that in Group A （P 〈 0.05 or P 〈 0.01 ）, but showed insignificant difference in comparing the Group A with all the treated Groups on d 14 （P〉0. 05）. Conclusion LS and CM ＋ LS could inhibit the growth and metastases of LLC, and shows inhibition on CD44 expression in tumor cells and P-selectin level in serum, which may be one of the mechanisms of LS in suppressing tumor metastasis.