3,4-二羟基苯乙酮对老年大鼠血小板膜磷脂成分的影响

DHAP100mg·kg-1·d^-1,po,持续10周,可使老龄大鼠血小板膜磷脂(PL)含量增加,膜磷脂与膜胆固醇比值(PL/C)相对增高;血小板磷脂成分——磷脂酰乙醇胺(PE)和磷脂酰胆碱(PC)含量也有明显增加(P<0.01),但血小板膜总脂及膜胆固醇含量无明显变化(P>0.05)。本实验结果提示:DHAP对血小板功能的影响可能与其抑制血小板膜磷脂降解、改变血小板膜组分有关。

EFFECT OF 3,4-DIHYDROXYACETOPHENONEONE ON PHOSPHOLIPID COMPONENTS OF PLATELET MEMBRANE IN AGED RATS

3,4-Dihydroxyacetophenone(DHAP)is one of the constituents of Ilex pubescence Hook.et Arn.var.glaber Chang.The effects of DHAP on the composition of blood plateletmembrane in aged rats was examined.No change of total lipid and cholesterol contents was observedin aged rats.While a slight but significant decrease of phospholipid in platelet membrane was found,which lead to diminution of the ratio of phospholipid to cholesterol.The contents ofphosphatidylethanolamine and phosphatidylcholine decreased markedly by as less as 23.0%and 19%,respectively ,compared with the young group.Whereas the degree of reduction of phospholipid ,theratio of phospholipid to cholesterol,phosphatidylethanolamine and phosphatidylcholine weresignificantly lightened after irrigation with DHAP 100 mg· kg^-1 for 10 weeks.In the treatmentgroup ,the values of phosphatidylethanolamine and phosphatidylcholine of platelet membrane werereduced by 8.0%and 10.5%, respectively,vs the group of aged rats.It is concluded that reducedphospholipid hydrolysis is involved in mediating DHAP inhibition of platelet function.