海南粗榧新碱衍生物HH07A对体外L1210细胞的杀伤作用

体外培养的小鼠L1210细胞被HH07A2μg·ml^-1作用24h后,与对照组细胞相比,其细胞数不再增长,有丝分裂数及集落形成率下降,细胞形态及细胞周期动力学均发生一定的变化。且HH07A大剂量短期作用抑制Ll210细胞集落形成的效率高于低剂量持续作用。

CYTOCIDAL EFFECT OF HH07A, A DERIVATIVE OF HAINANENSINE,ON L1210 CELLS IN VITRO

The L1210 cell rapidly ceased to proliferate and its mitotic index decreased markedly after being exposed to HH07A 2μg·ml^-1 during exponential growth phase. However,34.6% of the cells were still able to form colonies in soft agar if HH07A was removed after 24 h ofincubation( the colony-forming efficiency for control cells was 63.7%)and the cell viabilityremained at about 94%.The DNA contents in L1210 cells were measured with a flow-cytometer.Theresults showed that the cell-cycle was blocked at the S phase and the number of cells in G₂+Mphasedecreased significantly.The time response course for L1210 cells indicated that the inhibitory effect of HH07A on L1210 clonogenic cells was slightly time dependent.