牛膝多糖硫酸酯的合成及其抗病毒活性

从中药牛膝中提取得到的一种新多糖化合物一牛膝多糖,用不同的硫酸化试剂合成牛膝多糖硫酸酯。研究了最佳反应条件,其产物水溶性好,呈中性,最高产率达82.11%,取代度(Ds)···达到三以上,硫含量达21%以上。根据牛膝多糖的分子结构,多糖分子中的羟基已最大限度地被酯化。体外抗病毒试验初步表明牛膝多糖硫酸酯有很强的抑制乙型肝炎病毒HBsAg和HBeAg的活性,对I型单纯性疱疹病毒也有明显的抑制力。

SYNTHESIS OF ACHYRANTHES BIDENTATA POLYSACCHARIDE SULFATE AND ITS ANTIVIRUS ACTIVITY

Sulfation of Achyranthes bidentata polysaccharide (Abps)with sulfuric acid or sulfurtrioxide-pyridine or chlorosulfonic acid-pyridine was studied.A homogeneous sulfation method withgood yield of 82.11%was obtained, using chlorosulfonic acid in an excess of pyridine. SulfatedAchyranthes bidentata polyaccharide was obtained as an amorphous sodium salt easily souluble in water. The UV and IR specttum of Abps sulfate showed absorptions at 208, 268, 286 nm and 1232,823. 6 cm^-1 respectively. The sulfur content of the products was found to be 20～22%. The degreeof suhtitution varied from 2. 8 to 3.2. It showed that the hydroxy group of Abps was almostcompletely esterified by chlorosulfonic acid. The Abps sulfate was shown to have high activity as anti-HBsAg and HBeAg. It is also effectiveon simple herpes virus type-I.