在脉冲电流作用下胰岛素经皮吸收对糖尿病大鼠血糖的影响

实验结果表明,在一定范围内糖尿病大鼠血糖下降的幅度与脉冲电流强度、频率及治疗时间成正比,但当脉冲电流强度超过0.8mA/cm2,频率超过3000Hz时,糖尿病大鼠血糖下降的幅度不再继续增加,即应用较小的电流强度及脉冲频率就能有效地促进胰岛素透皮吸收。同时脉冲电流的占空比为1:l时,糖尿病大鼠血糖下降的幅度最大。为使糖尿病大鼠血糖下降的幅度基本相当,皮下注射与经皮吸收方式给药所用胰岛素的剂量约为1:16。

EFFECT OF PULSE CURRENT IONTOPHORETIC TRANSDERMAL DELIVERY OF INSULIN ON BLOOD GLUCOSE IN DIABETIC RATS

ontophoresis of pulse current with various current intensity ,frequency , on/off ratio and duration of treatment was used to facilitate the transdermal delivery of insulin in order to control blood glucose levels in diabetic rats. Male Spraque-Dawley rats were made diabetic by ip injection of streptozotocin (65 mg·kg^-1 body weight).After two days, diabetic rats were anaesthe-tized with urethane (as 25%aqueous solution,1.5 mg·g^-1).Both thereservoir electrodes and thereceptor electrodes were applied on the abdominal site of the diabetic rats.Iontophoresis was carriedout using a prototype transdermal periodic iontophoretic system in order to provide the required directcurrent with desired pulse modes.The extent of reduction of blood gl ucose levels was found to bepositively correlated with the current intensity ,frequency and duration of treatment to some extent。But when the current intensity was over 0. 8 mA/cm² and the frequency was over 3000 Hz,thereduction of blood glucose levels did not continuously increase, Blood glucose levels were found to be better controlled when the on/off ratio of l :l was used.