丹酚酸A对小鼠脑缺血再灌注致学习记忆功能障碍的改善作用及作用机制

本实验采用脑缺血再灌注损伤造成小鼠学习记忆功能障碍模型。通过跳台和避暗实验法研究了丹酚酸对记忆获得功能障碍的改善作用。结果表明丹酚酸A3,10mg·kg^-1iv可以减轻脑缺血再灌注导致的小鼠学习记忆功能障碍状态。并使小鼠脑缺血再灌注后脑组织中脂质过氧化产物MDA含量明显降低。体外实验表明丹酚酸A10-8～10-7’mol·L^-1可以抑制大鼠脑组织匀浆脂质过氧化反应和羟自由基(·OH)的生成,表现出较强的抗氧化作用,提示丹酚酸改善脑缺血再灌注损伤的主要机制可能与其抗氧化作用有关。

PROTECTIVE EFFECTS OF SALVIANOLIC ACIDA AGAINST IMPAIRMENT OF MEMORY INDUCED BY CEREBRAL ISCHEMIA-REPERFUSION IN MICE

In the present experiments,an impairment of memory model was made by cerebral ischemia-reperefusion in mice.Sal A at the dosage of 3 and 10 mg·kg^-1iv was shown to improve the impairment of memory function induced by cerebral ischemia-reperefusion in step down and step through tests.In these tests,the number of errors of Sal A treated group was less and the latency was longer than that of control group.Meanwhile,Sal A 3 and 10 mg·kg-l iv was found to reduce the MDA contents in the cortex, hippocampus and striatum of cerebral ischemia-reperfused rats in vivo.Sal A 10～100 nmol·L^-1 was shown to inhibit the brain lipid-peroxidation and scavenge the free hydroxyl radical in vivo, These results indicate that the antagonistic effects of Sal A on impairment of learning and memory caused by cerebral ischemia-reperefusion may be related with its anti-oxidant activity.