耳针与补肾食疗对去势后骨质疏松症雌鼠骨密度和甲状腺功能变化的影响

目的：验证耳穴和补肾食疗防治骨质疏松症的疗效并探讨其治疗机理。方法：将切除6月龄雌鼠双侧卵巢建立骨质疏松症的动物模型，以假手术组和雌二醇组作为对照组。耳针组、补肾食疗组、耳针 补肾食疗组、雌二醇组分别经耳针、补肾补肾食疗和雌二醇的3个月治疗。用放免法测定大鼠血清的E2、T3、T4、TSH、PTH和骨密度、骨矿物含量。结果及结论：耳针、“补肾”食疗、雌二醇的治疗均能提高去势雌鼠的骨密度、骨矿物含量。其机理可能通过提高去势雌鼠的雌激素水平，调整垂体-甲状腺轴功能，从而抑制骨吸收，促进骨形成，降低骨转换率，纠正骨代谢的负平衡。

The effect of ear-acupuncture and nourishing kidney diet on bone density and thyroid function of osteoporosis in orariectiomized rats

Objective: To test and verify the therapeutic effect of ear-acupuncture and nourishing kidney diet on prevention and treatment of osteoporosis and explore its therapeutic mechanism. Method:Osteoporotic model was established by resecting bilateral of ovavies in 6-month-old female rats and then the animals was divided into estraditol group(EG), ear-acupuncture group(EAG), nourishing kidney diet group (EDG), ear-acupunctuer plus nourishing kidney diet group (ENDG) and untreated group(UG) at libertum, Sham-operated group (SOG) was also made, EAG,EDG,ENDG and EG was treated respectively with ear-acupuncture (EA), herb-diet(HD), ear-acupuncture with herb diet (EAHD) and estraditol benzoate (EB) for three month. By then, the rats were sacrificed. The blood was ellected. E 2, T 3, T 4, thyroid stimulating hormone(TSH), parathyroid hormone(PTH), bone mineral density, bone mineral contant, of serum were determined by RIA. Result: The contents of E 2, BMD, BMC, in the serum were increased and the secretion of T 3, T 4,TSH, PTH were inhibited in EGA?EDG?ENDG and EG comparing with UG. Conclusion:EAHD can promote BMC and BMD in ovariectomized rats. So they can probably be used to prevent and treat postmentopausal osteoporosis(PMO). Their mechanism may be the promotion of serum estrogen levels in ovariectomized rats. They also adjust the functional varies of hypophysis-thyriod axis. The final effects might be the depression of bone resorption, the increase of bone reformation and the decrease of bone reabsorption, then, the imbalance of bone metabolism were adjusted.