Methods for culture ofBombyx mori wing discs |
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| Authors: | Hideki Kawasaki |
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| Affiliation: | (1) Faculty of Agriculture, Utsunomiya University, Mine 350, Utsunomiya, Tochigi, Japan |
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| Abstract: | Summary A culture method forBombyx wing discs is described. Grace's insect medium,Bombyx hemolymph, and moderate doses of ecdysterone (0.02 to 0.1 µg/ml) were necessary for complete imaginal differentiation ofBombyx wing discs. This procedure is useful for understanding ecdysteroid action on insect imaginal differentiation and metamorphosis. |
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| Keywords: | wing disc culture imaginal differentiation |
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