鳖甲煎丸对肾间质纤维化模型大鼠肾脏的保护作用

目的观察鳖甲煎丸对肾间质纤维化实验大鼠肾脏的保护作用并探讨其作用机理。方法采用单侧输尿管结扎的方法复制大鼠肾梗阻模型,将其随机分为缬沙坦组、鳖甲煎丸组与模型组,并设假手术组。观察梗阻侧肾脏肾上腺髓质素(ADM)在蛋白及基因水平的表达,并测定其在肾组织中蛋白含量。结果模型组及各治疗组大鼠肾脏中肾上腺髓质素组织蛋白含量、蛋白表达以及mRNA的基因表达较假手术组明显降低,鳖甲煎丸组、缬沙坦组的含量及表达均高于模型组(P<0.05?,但两组之间比较无显著性差异。结论鳖甲煎丸能够明显上调肾间质纤维化大鼠肾脏ADM的蛋白及mRNA的表达,对肾脏起到保护作用。

Protective effect of Biejiajian Pills on kidney in rat model of renal interstitial fibrosis

Objective To observe the protective effect of Biejiajian Pills on experimental rat kidney from renal interstitial fibrosis and discuss the mechanism.Method The model of renal obstruction was copied in rats by unilateral ureteral obstruction(UUO) and then the rats were divided into UUOV group(treated with Valsartan),UUOB group(treated with Biejiajian Pills),model group and sham-operation group.The protein and gene expressions and content of adrenomedullin(ADM) in obstructed kidney were observed and determined.Result In the model group and UUOV,UUOB groups the content of protein and expressions of mRNA and ADM protein decreased obviously than those in the sham-operation group,and those in UUOB and UUOV groups were higher than those in the model group(P<0.05) but there was no significant difference between UUOB and UUOV groups.Conclusion Biejiajian Pills can up-regulate ADM protein and mRNA expressions in rats with renal interstitial fibrosis to protect the kidney.