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| 丹参酮ⅡA对四氯化碳损伤原代培养大鼠肝细胞的影响 | |
| 引用本文： | 刘永忠,王晓东,刘永刚.丹参酮ⅡA对四氯化碳损伤原代培养大鼠肝细胞的影响[J].中药材,2003,26(6):415-417. |
| 作者姓名： | 刘永忠 王晓东 刘永刚 |
| 作者单位： | 1. 西安市中心医院,西安,710003 2. 广州市花城制药厂,广州,510555 3. 第一军医大学中医系,广州,510515 |
| 摘 要： | 目的：研究丹参酮ⅡA对四氯化碳(CCl4)损伤原代培养大鼠肝细胞的保护作用。方法：原位灌流分离大鼠肝细胞培养36h后加入丹参酮ⅡA，同时造成CCl4损伤，于损伤3h测培养液中丙二醛(MDA)、一氧化氮(NO)的含量，谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)的活性，并用MTT法测肝细胞存活率。结果：丹参酮ⅡA明显改善细胞存活率，抑制CCl4引起的ALT、LDH活力的升高，同时抑制MDA、NO的产生及提高SOD活力。结论：丹参酮ⅡA能有效抑制CCl4造成的原代培养大鼠肝细胞损伤。 |
| 关 键 词： | 丹参酮ⅡA 四氯化碳损伤 原代培养 大鼠 肝细胞 |
| 修稿时间： | 2003年1月28日 |
Protective Effects of Tanshinone ⅡA on Injuried Primary Cultured Rat Hepatocytes Induced by CCl4 |
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| Liu Yongzhong ,Wang Xiaodong ,Liu Yonggang.Protective Effects of Tanshinone ⅡA on Injuried Primary Cultured Rat Hepatocytes Induced by CCl4[J].Jorunal of Chinese Medicinal Materials,2003,26(6):415-417. | |
| Authors: | Liu Yongzhong Wang Xiaodong Liu Yonggang |
| Institution: | Center Hospital of Xi'an, Xi'an 710003. |
| Abstract: | OBJECTIVE: To investigate the effect of tanshinone IIA on carbon tetrachloridlde (CCl4) intoxicated primary cultured rat hepatocytes. METHODS: The rat hepatocytes were separated by perfusion with collagenase solution. Tanshinone IIA was added to the cultured medium after incubating the rat hepatocytes for 36 hours. At the same time, CCl4 was added to induce hepatocytes injury for 1 h. The levels of ALT, LDH, NO, SOD, MDA and cell viability after cultivation for 3 h were determined respectively. RESULT: Tanshinone IIA significantly improved cell viability, inhibited the decrease of SOD activity and the increase of ALT, LDH, MDA, NO levels CONCLUSION: Tanshinone IIA protected effectively the primary cultured rat hepatocytes against CCl4-induced injury. |
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