不同剂量生白术对慢传输型便秘大鼠胃肠道传输功能及 Cajal 间质细胞的影响

目的：比较不同剂量生白术对慢传输型便秘大鼠（STC）胃肠道传输功能及 Cajal 间质细胞（ICC）的影响。方法 Wistar 大鼠40只，分为空白对照组、模型组、常规剂量生白术组、大剂量生白术组。空白对照组及模型组给予生理盐水灌胃，其余各组均给予相应药物灌胃治疗，14 d 后采用营养性半固体糊灌胃法测定各组大鼠胃残留率、黑色炭末推进率，并采用免疫组化法观察各组大鼠胃窦、小肠、结肠组织 ICC 细胞数量及形态的变化。结果常规剂量生白术组、大剂量生白术组与模型组比较，胃残留率明显降低（P ﹤0.05），而二者之间比较无统计学意义（P ﹥0.05）。常规剂量生白术组、大剂量生白术组均可增加黑色炭末推进率（P ﹤0.05），其中大剂量生白术效果优于常规剂量生白术（P ﹤0.05）。常规剂量生白术组、大剂量生白术组均可增加 c-kit阳性细胞的面积（P ﹤0.05），而大剂量生白术优于常规剂量生白术（P ﹤0.05）。结论生白术常规剂量及大剂量均可促进慢传输型便秘大鼠的胃动力，而在改善其肠道传输功能及增加 c-kit 阳性细胞面积方面则以大剂量白术效果为佳。

Atractylodes macrocephala Koidz on gastrointestinal trace and ICC in rat with slow transit constipation

Objective To observe the influence of Atractylodes macrocephala Koidz on gastrointestinal trace and ICC in rat with slow transit constipation. Methods The 40 Wistar rats were randomly divided into blank control group,model group,Atractylodes macrocephala Koidz normal-dose group,Atractylodes macrocephala Koidz high-dose group. The blank control and model group were given normal saline by gavage,the rest of the groups were given drug treatment accordingly. After 14 days,gastric motility and transit functions of intestinal movement were examined by ad-ministering intragastricly of semi-solid nutrition paste. The ICC changes in terms of the number and volume were ob-served by immunohistochemical method. Results Atractylodes macrocephala Koidz treatment group compared with model group can significantly reduce the gastric residual rate( P ﹤ 0. 05),but there was no significant difference with each other( P ﹥ 0. 05). Atractylodes macrocephala Koidz normal-dose and high-dose group can increase the carbon black transit rate(P ﹤ 0. 05),and Atractylodes macrocephala Koidz high-dose group were significantly higher than in the Atractylodes macrocephala Koidz normal-dose t group( P ﹤ 0. 05). After treatment by Atractylodes macrocephala Koidz,the volume of c-kit positive cell in colon were increased( P ﹤ 0. 05),and Atractylodes macrocephala Koidz high-dose group was more larger than the Atractylodes macrocephala Koidz normal-dose group(P ﹤ 0. 05). Conclusion The Atractylodes macrocephala Koidz normal-dose and high-dose group can promote the gastrointestinal motility,At-ractylodes macrocephala Koidz of high-dose has the better effect on transit function of intestinal movement and the vol-ume recovery of ICC in colon.