重症急性胰腺炎肺损伤大鼠sICAM-1水平变化及其意义

目的:探讨血清可溶性细胞间粘附分子-1(sICAM-1)水平变化与重症急性胰腺炎(SAP)肺损伤的关系.方法:SD大鼠采用逆行胰胆管注射30 g/L牛磺胆酸钠方法制作SAP模型取0,3,6,12,24 h不同时点血清进行sICAM-1检测,同时检测肺组织髓过氧化物酶活性.结果:制模3h后,血清sICAM-1水平开始持续上升(34.90-38.99μmol/L),肺组织髓过氧化物酶(MPO)活性在6 h后明显增强(P<0.05),血清sICAM-1水平与肺组织MPO活性呈正相关(r =0.897).结论:重症急性胰腺炎时血清sICAM-1水平的变化可能与肺部损伤程度密切相关.

Significance of concentration changes of serum soluble ICAM-1 in lung injury associated with severe acute pancreatitis in rats

AIM:To investigate the relationship between concentration changes of soluble intercellular adhesion molecule-1 (sICAM-1) in severe acute pancreatitis (SAP) and associated lung injury in rat. METHODS:A Rat SAP model was established by retrograde injection of 30 g/L sodium tau- rocholate into bile-pancreatic duct.Rats were sacrificed at 0,3,6,12,24 hours and their sera and left lungs collected.Sera sICAM-1 and my- eloperoxidase (MPO) activity in lung tissue were measured. RESULTS:Increases in sera sICAM-1 concen- trations were evident at 3 hours after SAP in- duced,and the MPO activity of lung tissue was significantly increased at 6 hours.Sera sICAM-1 concentration positively correlation with MPO activity (r=0.897). CONCLUSION:The concentration change of serum sICAM-1 is associated with the pathologi- cal injury magnitude of lung in rat severe acute pancreatitis.