| 斑马鱼消化器官发育缺陷突变体的遗传筛选 |
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| 引用本文： | 黄超,张冲,蒋发明,王飞,阮华,黄红辉.斑马鱼消化器官发育缺陷突变体的遗传筛选[J].实验动物与比较医学,2015,23(5):441-445. |
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| 作者姓名： | 黄超 张冲 蒋发明 王飞 阮华 黄红辉 |
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| 作者单位： | 西南大学,西南大学,西南大学,西南大学,西南大学,西南大学 |
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| 基金项目： | 国家自然科学基金面上项目（31471365）、西南大学中央高校基本科研业务费（XDJK2014A013、2362014xk02） |
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| 摘 要： | 摘要] 目的 正向遗传筛选斑马鱼肝脏、肠和胆囊发育缺陷突变体。方法 ENU诱变野生型斑马鱼并开展经典的F2代筛选，以lfabp为探针的全胚原位杂交、BES-H2O2-Ac荧光染料分别检测斑马鱼早期胚胎肝脏、肠和胆囊的表型。结果 在128个突变基因组中筛选获得了源自14个F2家族的斑马鱼消化器官发育缺陷突变体品系23个，并按表型划分为6类。结论 斑马鱼肝脏、肠和胆囊的发育调控机制有相似性和差异性。
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| 关 键 词： | 斑马鱼 消化器官 遗传筛选 突变体 |
| 收稿时间： | 2015/6/22 0:00:00 |
| 修稿时间： | 2015/6/24 0:00:00 |
Genetic screening of zebrafish mutants with developmental defects in the digestive organs |
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| HUANG Chao,ZHANG Chong,JIANG Fa-ming,WANG Fei,RUAN Hua and HUANG Hong-hui.Genetic screening of zebrafish mutants with developmental defects in the digestive organs[J].Laboratory Animal and Comparative Medicine,2015,23(5):441-445. |
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| Authors: | HUANG Chao ZHANG Chong JIANG Fa-ming WANG Fei RUAN Hua and HUANG Hong-hui |
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| Institution: | Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China;Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environments and Bio-Resources of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China |
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| Abstract: | Abstract] Objective To obtain zebrafish mutants with developmental defects in digestive organs from a forward genetic screen. Methods ENU mutagenesis and a classical F2 genetic screen were performed, RNA whole mount in-situ hybridization using lfabp as probe and BES-H2O2-Ac staining were applied to examine the liver, intestine and gall bladder phenotype in zebrafish embryos. Results We harvested 23 mutant lines with developmental defects in digestive organs (originated from 14 F2 families) after screening 128 mutagenized genomes. These mutants were classified into six groups according to phenotypes. Conclusions The liver, intestine and gall bladder share and differ in their developmental molecular mechanisms in zebrafish. |
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| Keywords: | Zebrafish Digestive organs Genetic screening Mutants |
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