去卵巢对骨质疏松大鼠皮肤衰老的影响

目的 用去卵巢大鼠构建的骨质疏松症模型是研究绝经后骨质疏松疾病的最适模型，然而大鼠卵巢去除后对皮肤的影响如何，这方面的研究甚少。本研究旨在观察去卵巢对大鼠皮肤衰老指标的影响。方法 3月龄健康SD雌性大鼠20只，随机分为假手术组（Sham组）和去卵巢组（OVX组），卵巢去除后3个月，测定血清雌二醇，以及皮肤组织中与衰老相关的生化指标如过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性以及丙二醛（MDA）、羟脯氨酸（HYP）、脂褐质（LF）含量，并观察皮肤组织病理学变化，用计算机图像分析系统定量分析。结果 OVX组大鼠血清雌二醇水平显著降低；组织病理学观察显示OVX组表皮、真皮厚度较Sham组显著变薄（P<0.01），皮肤胶原纤维减少，排列疏松；与Sham组相比，OVX组大鼠皮肤CAT、GSH-Px和SOD活性显著降低（P<0.01），MDA含量较Sham组明显增加（P<0.01），皮肤组织中HYP含量明显减少，LF含量显著增多（P<0.01）。结论 3月龄大鼠卵巢去除后3个月可导致皮肤衰老，可能是研究绝经后皮肤衰老的适宜模型。

Effect of ovariectomy on the skin aging in rats with osteoporosis

Objective Ovariectomized rats are the most suitable model for the study of postmenopausal osteoporosis. However, the effect of ovariectomy on rat skin is little studied. The aim of this study was to observe the effect of ovariectomy on the skin aging indexes in rats. Methods 20 healthy 3-month-old female SD rats were randomly divided into sham group and ovariectomized (OVX) group. Serum estradiol was detected at 3 months after ovariectomy, the skin aging-related biochemical indexes such as catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity and the content of malondialdehyde (MDA), hydroxyproline (HYP) and lipofuscin (LF) were also detected. The structure of skin tissue was observed and detected using a pathological image analysis system. Results The serum E₂ of OVX rats was significantly lower than that in the sham group, the thickness of epidermis and dermis of OVX group was significantly thinner than that of the sham group (P<0.01), the amount of collagen fibers was decreased and their arrangement was very loose. Compared with the sham group, the activity of CAT, GSH-Px and SOD in the OVX group was significantly decreased (P<0.01), and the content of MDA was significantly increased (P<0.01). The content of HYP in skin tissue was significantly decreased, and LF content was significantly increased (P<0.01) in the OVX group. Conclusions Skin aging can be induced at 3 months after ovariectomy in 3-month old rats, which may be a good model for the study of skin aging in postmenopausal women.