| 长爪沙鼠体外受精与早期胚胎体外培养体系的初步建立 |
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| 引用本文： | 郭红刚,李莉,周生来,卢领群,李坤,杜江涛,石巧娟,金秀清,李长龙,萨晓婴,应华忠,褚晓峰. 长爪沙鼠体外受精与早期胚胎体外培养体系的初步建立[J]. 实验动物与比较医学, 2017, 25(6): 624-631 |
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| 作者姓名： | 郭红刚 李莉 周生来 卢领群 李坤 杜江涛 石巧娟 金秀清 李长龙 萨晓婴 应华忠 褚晓峰 |
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| 作者单位： | 浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,杭州市疾病预防控制中心, 杭州 3100212,中国医科大学实验动物部, 沈阳 110001,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,首都医科大学基础医学院, 北京 100069,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013,浙江省医学科学院, 浙江省实验动物与安全性研究重点实验室, 杭州 310013 |
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| 基金项目： | 国家科技支撑计划子课题（No.2015BA109B01-02）；浙江省级公益性技术应用研究计划（No.2015C37119）；浙江省卫生厅课题（No.2015KYB093）。 |
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| 摘 要： | 目的 探讨自配的获能液和受精液用于长爪沙鼠体外受精的可行性，为长爪沙鼠的胚胎保种提供参考。方法 用自配的获能液和受精液对长爪沙鼠进行体外受精，并用改良后的KSOM培养液对长爪沙鼠的2细胞胚胎进行体外培养试验。结果 长爪沙鼠体外受精率在60%以上，沙鼠2细胞胚胎能够在体外进一步发育。结论 初步建立了长爪沙鼠体外受精和胚胎发育体系，但需要进一步优化。
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| 关 键 词： | 长爪沙鼠 体外受精 体外培养 |
| 收稿时间： | 2017-06-07 |
Establishment of a modified in vitro mongolian gerbil oocyte fertilization and embryo culture system |
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| GUO Hong-gang,LI Li,ZHOU Sheng-lai,LU Ling-qun,LI Kun,DU Jiang-tao,SHI Qiao-juan,JIN Xiu-qing,LI Chang-long,SA Xiao-ying,YING Hua-zhong and CHU Xiao-feng. Establishment of a modified in vitro mongolian gerbil oocyte fertilization and embryo culture system[J]. Laboratory Animal and Comparative Medicine, 2017, 25(6): 624-631 |
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| Authors: | GUO Hong-gang LI Li ZHOU Sheng-lai LU Ling-qun LI Kun DU Jiang-tao SHI Qiao-juan JIN Xiu-qing LI Chang-long SA Xiao-ying YING Hua-zhong CHU Xiao-feng |
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| Affiliation: | Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Hangzhou Disease Prevention and Control Center, Hangzhou 3100212,Experimental Animal Department of China Medical University, Shenyang 110001,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Capital Medical University Basic Medical College, Beijing 100069,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China,Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China and Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou 310013, China |
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| Abstract: | Objective To investigate the feasibility of self-made capacitation liquid for in vitro fertilization of Mongolian gerbils,and to provide a reference for gerbil embryo cryopreservation.Methods In vitro fertilization of Mongolian gerbil was performed with the self-prepared capacitation solution and semen,and the 2-cell embryos of Mongolian gerbils were cultured in vitro using an improved KSOM culture medium.Results The in vitro fertilization rate of gerbils was over 60%,and some gerbil 2-cell embryos could develop further in vitro.Conclusions An in vitro fertilization and embryo development system of Mongolian gerbil has been established,but it needs further optimization. |
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| Keywords: | Mongolian gerbil In vitro fertilization In vitro culture |
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