系统性念珠菌感染小鼠模型的制备

目的 建立稳定的系统性念珠菌感染小鼠模型，规范其操作方法。方法 采用环磷酰胺对小鼠进行免疫抑制后，选择白色念珠菌（C.albicans）和非白念珠菌（C.parapsilosis）分别接种ICR小鼠，从免疫抑制、菌株制备、接种剂量和接种途径等方面对建模过程进行质量控制，通过生存分析、组织载菌量和病理学检查对模型进行评价。结果 我们建立的系统性念珠菌感染小鼠模型显示肾脏为靶器官，多器官弥散性真菌感染的典型病理组织学改变。结论 通过对建模过程各环节进行规范，可得到稳定的系统性念珠菌感染小鼠模型，该模型可应用于系统性念珠菌感染的致病机理，免疫防御及抗真菌药物筛选等研究领域。

Preparation of a murine model of systemic Candida albicans infection

To establish a stable mouse model of systemic Candida infection and to set up related standard operation procedure. Methods ICR mice were infected with C. albicans or C. parapsilosis by tail vein injection after immunosuppression by cyclophosphamide. The quality control key points included immunosuppression, strain preparation, inoculation doses and the route of inoculation. Survival analysis, bacterial loads and pathological examination were performed to evaluate the prepared model. Results The developed model showed fugal-specific lesions in multiple organs, especially in the kidneys revealed by histopathological examination. Conclusions A stable mouse model of systemic Candida albicans infection can be successfully established by following standardized operation procedure. This mouse model may provide a useful tool for studies on pathogenesis and immune defense of fungal infection and new anti-fungal drug development and so on.