实验动物中沙门菌的实验室检测能力验证的结果与分析

目的 通过实验动物中沙门菌检测能力验证计划,了解实验动物检测机构对沙门菌的检验能力,提高实验动物质量检测水平。方法 按照CNAS批准的能力验证方案,通过冷冻干燥法制备含有沙门菌及干扰菌的实验动物粪便样品,经过稳定性和均匀性检验合格,作为能力验证样品。采用随机编号,经冷链运输发放给参加单位,并附作业指导书。在规定时限提交检验报告和原始记录复印件,其结果与样品预检结果一致的判为满意结果,不一致或未能提交结果的判为不满意结果。结果 全国共有20个省市的30个实验室参加沙门菌能力验证项目,其中28个实验室获满意结果,占总参加机构的93.3%,不满意的2个实验室,占6.7%。采用分离培养方法的有29个实验室,采用PCR方法的有2个实验室。结论 实验动物质量检测机构沙门菌检测能力较高,实施能力验证计划能够反映实验室的检测水平。

Results and analysis of the proficiency of laboratories in detection of Salmonella in laboratory animals

Objective To understand the Salmonella detectability in the laboratory animal testing laboratories, improve the level of detection for the quality of laboratory animals, by means of laboratory animals Salmonellaproficiency testing program. Method According to the proficiency testing program approved by CNAS, freeze-dried animal stool samples containing Salmonella bacteria and interference bacteria were prepared, and through stability and homogeneity tests qualified as proficiency testing samples. The randomly numbered samples were issued to the participating units by cold-chain transportation, and attached work instructions. The original reports and copies of the tests should be submitted on time. The sample results consistent with the results of the pretested results were considered as satisfactory, and the results inconsistent or fails to submit were judged as unsatisfactory. Results A total of 30 laboratories from 20 provinces and cities nationwide participated in this proficiency testing programs for Salmonella, including 28 (93.3%) laboratories with satisfactory results, and two laboratories unsatisfactory (6.7%). 29 laboratories used separate culture methods, and two laboratories used PCR method. Conclusions The laboratory animal quality inspection agencies have good detection ability for Salmonella. The implementation of the capacity verification plan can well reflect the detection level of laboratories.