肝螺杆菌感染对BALB/c小鼠免疫应答干扰的初步研究

[摘要] 目的 探讨肝螺杆菌（Helicobacter hepaticus,H.hepaticus）感染对小鼠骨髓源树突状细胞（DC）表面分子形态和机体免疫应答的干扰。方法 以H.hepaticus（ATCC 51450）灌饲SPF级BALB/c雄性小鼠,于末次接种后5个月体外分离培养骨髓DC,经粒细胞-单核细胞集落刺激因子（GM-CSF）、白细胞介素-4（IL-4）,刺激DC增殖、分化,流式细胞仪分析DC细胞表面分子CD11c、CD40、CD80、MHCII的表达率。此基础上,用新城疫病毒（NDV）ZJ1株人工接种实验组和对照组小鼠,每周测定NDV血清抗体效价,比较抗体产生的差异。结果 实验组MHC II和CD40分子的表达率高于对照组。NDV抗体水平第1周实验组略低于对照组;第2至第5周内实验组小鼠的抗体水平均高于对照组,差异有显著性;第6周两组小鼠血清抗体均呈下降趋势,差异无显著性。结论 H.hepaticus感染对小鼠骨髓DC成熟有促进作用,能提高MHC II和CD40表达水平,可促进BALB/c小鼠产生抗NDV的抗体水平。

Preliminary observation on the interference effect of Helicobacter hepaticus infection on the immune response in BALB/c mice

[Abstract] ObjectiveTo determine the interference of H.hepaticusinfection on functional characteristics of dendritic cells (DC) surface molecules and the immune response in mice. Methods Male BALB/c mice were inoculated with H.hepaticus (ATCC 51450).Murine bone marrow-derived dendritic cells (DC) were isolated and co-cultured which were stimulated by GM-CSF and IL-4at the fifth month post last inoculation. Then DCs were subjected to FACS analysis for surface markers (CD11c, CD40, CD80 and MHCII) detection.On this basis, virussuspension of Newcastle disease virus(NDV) ZJ1 strain was inoculated into the mice. Serum was collected for detection of the NDV antibody titer in serum weekly toexplore the difference of antibody titer between two groups. Results The results showed that the expression rates of CD40 and MHCII on DCs of mice in experimental group were higher than that in control group. The NDV antibody titer of experimental group was slightly lower than the control group in the first week. During the 2nd to 5th weeks, the titer was higher than the control group, difference is very significant. In the 6th week, the titer of two groups both tended to fall. Conclusions H.hepaticus infection could promote bone marrow DC maturation in mice, stimulate the expression rates of MHC II and CD40, and enhance the NDV antibody levels.