奥沙利铂周围神经毒性模型大鼠行为学检测方法的比较

目的 进行两种大鼠机械性痛觉敏感性测量方法、两种冷刺激缩足反应测量方法的比较研究。方法 雌性Wistar大鼠,腹腔注射奥沙利铂4 mg/kg,建立标准奥沙利铂周围神经毒性大鼠模型。检测机械和冷热温度刺激下大鼠行为学变化,并对不同行为学检测方法的特点进行比较分析。结果 模型组大鼠较正常组相比50%缩足阈明显降低(P<0.05)、出现痛觉过敏及痛觉超敏(P<0.05)、冷刺激诱发的缩足次数增多(P<0.05),两组大鼠对热痛刺激反应均无明显差异,与临床患者症状表现基本一致。结论 Von Frey纤维丝刺激检测中4 g和15 g方法可更好的模拟临床患者的痛觉过敏、超敏反应;up-down法客观量化的反应出大鼠缩足阈值下降的程度。冷温度刺激中,丙酮喷洒法可较直观的观察大鼠受到冷刺激后的反应。

Comparison of different behavioral test methods of rat models of oxaliplatin-induced peripheral neurotoxicity

Objective To compared two different methods, the mechanical allodynia test and cold hyperalgesia test in a rat model of oxaliplatin-induced peripheral neuropathy. Methods Oxaliplatin frequently causes severe acute and chronic peripheral neuropathies. In this experimental study, 40 healthy female Wistar rats (body weigh 210±10 g) were used. We used Von Frey filament test to determine the 50% paw withdrawal threshold and mechanical allodynia in rats, and also used acetone spray and cold plate to examine the pain behavior in rats. Results Compared with the control group, the rats of model group showed a 50% decrease of the paw withdrawal threshold (P<0.05), appearance of allodynia and hyperalgesia (P<0.05), and increase of the number of paw withdrawals caused by cold stimulation (P<0.05), but there were no obvious differences in the response to heat stimulation in rats of both groups. Those results were similar in general to the clinical manifestations in patients.Conclusions Both the two ways of mechanical allodynia test are good choices. von Frey filaments with bending forces of 4 g and 15 g are a better way to simulate the test of mechanical allodynia and hyperalgesia in clinical patients, while the up-down method can objectively and quantitatively reflect the reduction of paw withdrawal threshold. The acetone spray test allows us to immediately record the reaction of rats after cold stimulation. Our findings may be useful for further studies of oxaliplatin-induced neuropathy.