miR-5572转基因小鼠构建病态窦房结综合征疾病模型的繁殖与鉴定

目的 探讨miR-5572转基因小鼠构建病态窦房结综合征疾病模型的可行性。方法 繁殖与鉴定了miR-5572 F1及F2代野生型纯合子及杂合子小鼠，并通过形态学、心电图记录及窦房结组织Cav1.2、Cav1.3 mRNA和蛋白表达水平测定来观察疾病模型。结果 F2代miR-5572纯合子敲入小鼠在形态上较野生型小鼠生长缓慢，体型较小。相较于杂合子和野生型小鼠，纯合小鼠的平均心率明显偏低（P<0.05），差异有显著性。miR-5572纯合子小鼠窦房结组织Cav1.2、Cav1.3 mRNA和蛋白表达水平低于野生型（P<0.05），差异有显著性。结论 过表达miR-5572转基因小鼠可以构建病态窦房结综合征疾病模型。

Breeding and genotyping of a miR-5572 transgenic mouse model of sick sinus syndrome

Objective This study aimed to explore the possibility of establishing a model of sick sinus syndrome by using miR-5572 transgenic mice. Methods F1 and F2 miR-5572 transgenic mice were bred and genotyped, and then observed the phenotype levels of miR-5572 transgenic mice by morphology, electrocardiogram record (ECG) and the Cav1.2 and Cav1.3 expressions levels of mRNA and protein in sinoatrial node tissue of homozygous, heterozygote and wild type mice.Results Compared with the wild type and heterozygous mice,the miR-5572 homozygous mice showed a development delay and smaller body shape,and had slower average heart rate.The mRNA and protein levels of Cav1.2 and Cav1.3 in the sinoatrial node tissues were significantly lower.Conclusions The results of this study indicate that miR-5572 homozygous mice may be an efficient approach to establish the model of sick sinus syndrome