| 复合刺激创伤后应激障碍模型大鼠重要脑区的病理变化 |
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| 引用本文： | 陈旺,李陈成,李森,冯东亮,洪有建,南伟,江龙,黄显凯,伍亚民. 复合刺激创伤后应激障碍模型大鼠重要脑区的病理变化[J]. 实验动物与比较医学, 2016, 24(1): 87-91 |
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| 作者姓名： | 陈旺 李陈成 李森 冯东亮 洪有建 南伟 江龙 黄显凯 伍亚民 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所创伤外科,第三军医大学大坪医院野战外科研究所创伤外科,第三军医大学大坪医院野战外科研究所第三研究室,甘肃省兰州市兰州大学第二临床医学院骨科,第三军医大学大坪医院野战外科研究所创伤外科,兰州大学第二临床医学院骨科,第三军医大学大坪医院野战外科研究所创伤外科,第三军医大学大坪医院创伤外科,第三军医大学大坪医院野战外科研究所第三研究室 |
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| 基金项目： | 全军医药卫生科研基金(08G096). |
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| 摘 要： | [摘要]目的 研究复合刺激PTSD模型大鼠重要脑区的病理变化。方法 成年健康SD雌性大鼠20只，随机分为正常组、模型组，每组10只。模型组按复合刺激方法建立PTSD大鼠模型，4W后对两组大鼠行高架十字迷宫和Morris水迷宫检测；行为检测结束后对大鼠大脑皮层和海马CA1、CA2、CA3区和齿状回进行HE染色和Nissl染色，观察其病理变化特点。结果 正常组大脑皮层和海马各区细胞形态规则、分布均匀，核仁及边界清晰，胞浆尼氏体丰富，无明显神经元变性坏死；模型组大脑皮层细胞形态较规则、分布均匀，无明显病理改变；海马CA1、CA3区细胞形态不规则，排列紊乱，细胞间隙增大，细胞数量减少，有较多空泡样细胞病变，CA3区更为明显；CA2区细胞排列较规则，分布较均匀；齿状回可见部分细胞排列疏松、细胞间隙增大、尼氏体数量减少。结论 PTSD模型大鼠海马CA1、CA3区和齿状回均有不同程度的病理改变，为探讨PTSD患者的病理机制提供了实验依据。
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| 关 键 词： | 创伤后应激障碍；大脑皮层；海马；病理学 |
| 收稿时间： | 2015-08-07 |
| 修稿时间： | 2015-08-31 |
Pathological changes in some important brain areas of rats with post-traumatic stress disorder induced by combined stress |
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| CHEN Wang,LI Chen-cheng,LI Sen,FENG Dong-liang,HONG You-jian,NAN Wei,JIANG Long,HUANG Xian-kai and WU Ya-min. Pathological changes in some important brain areas of rats with post-traumatic stress disorder induced by combined stress[J]. Laboratory Animal and Comparative Medicine, 2016, 24(1): 87-91 |
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| Authors: | CHEN Wang LI Chen-cheng LI Sen FENG Dong-liang HONG You-jian NAN Wei JIANG Long HUANG Xian-kai WU Ya-min |
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| Affiliation: | Department of Traumatology, Research Institute of Surgery/Daping Hospital, Third Military Medical University, Chongqing 400042, China;Third Department of Research Institute of Surgery/Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China;Department of Traumatology, Research Institute of Surgery/Daping Hospital, Third Military Medical University, Chongqing 400042, China;Third Department of Research Institute of Surgery/Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China;Third Department of Research Institute of Surgery/Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China;Department of Orthopedics, the Second Clinical Medical College of Lanzhou University, Lanzhou 730030;Department of Traumatology, Research Institute of Surgery/Daping Hospital, Third Military Medical University, Chongqing 400042, China;Third Department of Research Institute of Surgery/Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China;Department of Orthopedics, the Second Clinical Medical College of Lanzhou University, Lanzhou 730030;Department of Traumatology, Research Institute of Surgery/Daping Hospital, Third Military Medical University, Chongqing 400042, China;Third Department of Research Institute of Surgery/Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China;Department of Traumatology, Research Institute of Surgery/Daping Hospital, Third Military Medical University, Chongqing 400042, China;Third Department of Research Institute of Surgery/Daping Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400042, China |
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| Abstract: | Objective To study the pathological changes in some important brain areas of rats with post-traumatic stress disorder (PTSD) induced by combined stress.Methods Twenty healthy adult female SD rats were randomly divided into normal group and model group, with 10 rats in each group. The PTSD model was established by combined stress. After 4 weeks, the rat behaviors of the two groups were tested by elevated plus maze and Morris water maze. After the test, samples of the cerebral cortex, areas of CA1, CA2, CA3 and hippocampal dentate gyrus were taken for pathological examination using HE and Nissl staining. Results In the normal group, the cell morphology and distribution were regular and the cell nucleoli were clear in the cerebral cortex and hippocampus, cytoplasmic Nissl bodies were abundant, and there were no obvious neuronal degeneration and necrosis. In the model group, the cell morphology and distribution in the CA1 and CA3 areas were irregular, with increased cell gaps, and in addition, there were some pathological changes of physaliferous cells, especially in the CA3 area. The cell morphology and distribution in the CA2 area were regular. In the dentate gyrus, some cells arranged loosely, the cell gap was widened, and the amount of Nissl bodies was decreased. Conclusions There are some pathological changes in the hypocampal CA1, CA3 and dentate gyrus in rat models of post-traumatic stress disorder, and they will provide an experimental basis for studying the pathological mechanism of PTSD patients. |
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| Keywords: | Posttraumatic stress disorder Cerebral cortex Hippocampus Pathology |
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