无菌猪的制备与微生物质量控制

菌群与宿主相互关系等热点研究促进了对无菌动物需求的迅猛发展。无菌猪作为一种特殊的模式动物，其在科学研究中不仅排除了背景微生物因素的影响，而且与常用的无菌小鼠相比，其在解剖、生理、代谢等方面与人更为相似，因此在生命科学研究领域具有重要的应用价值。本文结合现有国外无菌猪研究进展和本实验室在无菌猪研究积累，就无菌猪的制备与微生物质量控制进行简要探讨。

Breeding and microbiological quality control of germ-free pigs

The industry of germ-free animals has been a hot spot in research along with the rapid development of studies on the relationship between microbiota and host diseases.Because it is pathogen-free,and the high degree of similarity in anatomy,physiology,pathogenesis to humans,germ-free pig is considered a clinical relevant model to be widely used in life science research.Based on the current state of research of germ-free pig cultivation at home and abroad and the experimental studies carried out in our laboratory as well,this article gives a simple discussion on germ-free technique of domestic pigs.