小鼠左肺原位移植模型的建立

目的：通过显微外科技术建立小鼠原位肺移植模型，为肺移植研究提供动物模型。方法：采用C57BL/6小鼠作为供、受体，行同基因小鼠原位左肺移植，使用Cuff套管法进行气管及血管吻合。术后7天、14天、21天、28天取移植肺及原肺，行HE染色，评价肺移植后效果。结果：学习曲线后, 共30例小鼠移植,手术成功率89%, 小鼠成活率100%。供体手术时间：35.2?.81min，受体手术时间：24.6?.42min，冷缺血时间是：46.6?.92min，热缺血时间是：17.2?.08min。同基因移植物大体及病理无明显改变，病理显示与原肺无差别。结论：本技术能够方便快捷建立小鼠肺移植模型，成功率高，可重复性强，符合原位肺移植临床生理，是研究肺移植发病机制和治疗的良好动物模型。

Establishment of a mouse model of orthotopic lung transplantation

Objective To investigate the lung transplantation by establishing an orthotopic mouse lung transplantation model by microsurgery. Methods Thirty SPF male C57BL/6 mice served as both recipient and donor. Orthotopic lung transplantation models were established in the mice by three-cuff anastomotic technique. Native and transplanted lung tissue samples were taken at 7, 14, 21 and 28 days after transplantation for pathological evaluation of the outcomes using HE staining. Results After learning curve, 30 mice received transplantation. All mice survived and the success rate of lung transplantation was 89%. The donor operation time was 35.2±9.81 min and recipient operation time was 24.6±7.42 min. Mean cold ischemia time was 46.6±8.92 min, and warm ischemia time was 17.2±3.08 min. No histological alterations were routinely detected in the isograft lungs at all times post-transplantation compared with the native lungs. Conclusions Mouse lung transplantation model can be successfully established by the established technique with a high success rate and excellent reproducibility, and is in accordance with the physiology of clinical orthotopic lung transplantation. It is a good animal model for studying the pathogenesis and treatment of lung transplantation.