野生小鼠来源1号染色体替换系小鼠的生长表型与血液生化检测

目的 分析野生小鼠来源1号染色体替换系小鼠群体的生长表型与血液生化指标，探讨替换系群体的QTL定位潜力。方法 以染色体替换系近交后代小鼠为研究对象，测定其体重、体长、尾长、血液生化、内脏器官重量等表型数据，统计其与C57BL/6差异显著性。结果 发现替换系小鼠在多个指标上与C57BL/6具有显著差异。外部表型上与C57BL/6相比，体重有显著差异的有括B6-Chr1KM和B6-Chr1TW，体长有显著差异的有B6-Chr1CM雌鼠和B6-Chr1SMX雄鼠，尾长有显著差异是所有替换系群体雌鼠；少部分品系在心脏、肾脏和脑部重量上存在与C57BL/6的显著差异（P＜0.05）；生化指标上，丙氨酸转移酶B6-Chr1CM雌鼠显著偏高，碱性磷酸酶B6-Chr1HZ雌鼠显著偏高、B6-Chr1KM雄鼠显著偏低，总胆红素B6-Chr1CM雄鼠、B6-Chr1SMX雄鼠和B6-Chr1HZ雄鼠显著偏高，甘油三酯B6-Chr1SMX雄鼠显著偏高，总胆固醇B6-Chr1TW雄鼠显著偏高。此外，B6-Chr1KM小鼠在体重、尾长、碱性磷酸酶、心脏重量、肾脏重量、脑部重量6个指标上与C57BL/6表现出有显著的差异，具有统计学意义（P＜0.05）。结论 培育的野生小鼠来源的1号染色体替换系群体在部分表型上与C57BL/6具有显著差异，具备作为QTL定位的遗传资源潜力。

Detection of growth phenotype and blood biochemical parameters of wild type-derived chromosome 1 substitution mouse strain

Objective To analyze the growth phenotype and blood biochemical parameters of chromosome 1 substitution mouse strain(CSS1), and investigate their potential of QTL mapping. Methods Body weight, body length, tail length, organ weight of the CCS1 mice were measured at different days to create a growth curve while blood biochemical indexes were measured at about the 80th day. Results The CCS1 mice were different from C57BL/6 mice in several indexes. Compared with the C57BL/6 mice during different developmental stages, six strains including B6-Chr1^KM mice were significantly different in body weight. There were five strains including B6-Chr1^CM mice significantly different with C57BL/6 mice in body length, and all of the CSS1 mice were significantly different from C57BL/6 mice in tail length. Part of CCS1 mice were significantly different from C57BL/6 mice in the weight of liver, spleen, kidney and brain. The ALT of female B6-Chr1^CM mice was significantly higher than that in the C57BL/6 mice. The ALP of female B6-Chr1^HZ mice was significantly higher than that in the male C57BL/6 and B6-Chr1^KM mice, and was significantly lower than that in the C57BL/6 mice. The TB of male B6-Chr1^CM, B6-Chr1^SMX and B6-Chr1^HZ mice was significantly higher than that of the C57BL/6 mice. The TG of male B6-Chr1^SMX mice and male B6-Chr1^TW mice was significantly higher than that in the C57BL/6 mice. Conclusions The phenotype of Chr1 CSS mice is quite different from commonly used inbred strain C57BL/6 mice. CCS1 mice show great potential in QTL mapping for their characteristic growth phenotype and blood biochemical indexes.