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A sandwich ELISA for detecting the hemagglutinin of avian influenza A (H10N8) virus |
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| Authors: | Lingling Chen Feier Ruan Mingbin Liu Jianfang Zhou Wenjun Song Kun Qin |
| Institution: | 1. Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang, China;2. Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang, China
National Institute for Viral Disease Control and Prevention, China CDC, Key Laboratory for Medical Virology, National Health Commission of P. R. China, Beijing, China;3. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China Nanchang Center for Disease Control and Prevention, Nanchang, Jiangxi, China;4. National Institute for Viral Disease Control and Prevention, China CDC, Key Laboratory for Medical Virology, National Health Commission of P. R. China, Beijing, China;5. State Key Laboratory of Respiratory Disease, Institute of Integration of Traditional and Western Medicine, Guangzhou Medical University, Guangzhou, China |
| Abstract: | Novel influenza A virus (H10N8) infected human with fatality in China during 2013-2014. It is important to detect such nonprevalent subtype influenza A virus in clinic and regular surveillance in the early stage for effective control and prevention from the potential pandemic. Unavailability of convenient rapid diagnosis for this subtype virus in resources-limited setting is an obstacle for timely recognizing human case. In the present study, a panel of mouse H10 specific monoclonal antibodies (mAbs) was generated, two of which were used to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting the hemagglutinin of avian influenza A (H10N8) virus. ELISA results showed high sensitivity with the lowest detection limit of 0.5HAU/50 μL for live virus, which laid a foundation for clinic use as a promising diagnostic methodology. |
| Keywords: | H10 subtype hemagglutinin monoclonal antibody sandwich ELISA |