结肠癌术后复发转移与抑癌基因启动子甲基化关系的研究

目的探讨结肠癌组织中,Syk、P16基因启动子甲基化与术后复发转移的关系。方法采用甲基化特异性PCR检测结肠癌组织及癌旁正常结肠组织的Syk、P16基因启动子甲基化状态,探讨结肠癌术后转移复发与Syk、P16基因启动子甲基化的关系。结果在60例结肠癌组织中,Syk、P16基因启动子甲基化发生率分别为38.3%（23/60）、42.0%（25/60）;在癌旁正常组织发生率分别为0（0/60）、18.3%（11/60）;在结肠癌组织中,Syk、P16基因甲基化发生率明显高于癌旁正常组织（P〈0.005）。在癌组织中检测到Syk启动子甲基化的23例中,有8例术后癌复发转移,发生率为34.8%（8/23）。而在癌组织中检测到P16基因甲基化的25例中,有11例术后发生复发转移,发生率为44.0%（11/25）。结论 （1）Syk、P16基因启动子甲基化与结肠癌术后复发转移密切相关。（2）结肠癌组织中Syk、P16基因启动子甲基化的检测有助于结肠癌术后复发转移的早期诊断及预后评估。

Study on the relationship between methylation of Syk,P16 gene and postopoerative recurrence in colon cancer

Objective To detect the relationship between methylation status of the promoter region of Syk,P16 gene in colon cancer,the relationship between the aberrant methylation of Syk,P16 gene and postoperative recurrence and metastasis after resection of colon cancer was analyzed.Methods By using methylation-specific polymerase chain reaction technique （MSP）, methylation of Syk, P16 promoter region from colon cancer patients （tumor tissues, adjacent normal tissues） were detected.To investigate the relationship between methylation status of the promoter region of Syk ,P16 gene and the postoperative recurrence and metastasis.Results The frequency of methylation of Syk、p16 promoter region was 38.3%（23/60）and 42.0%（25/60）in colon cancer.In contrast, The frequency of methylation of Syk、p16 promoter region was 0（0/60）and 18.3%（11/60）in adjacent normal tissues. the rate of methylation of Syk 、 p16 promoter in colon cancer was higher than that of adjacent normal tissues （P〈0.005）.The frequency of postoperative recurrence and metastasis in 23 cases with Syk promoter methylation was revealed in 34.8% （8/23） and that of p16 was in 44.0% （11/25）.A significant difference was noted between two groups.Conclusion First,Syk,p16 promoter methylation may play an important role in postoperative recurrence of colon cancer. Second,detecting the expression of syk,p16 gene in colon cancer could improve the early diagnosis of colon cancer and evaluate the prognosis.