| 细胞连续亲和-HPLC测定8种蟾蜍甾烯与MGC-803细胞亲和量及抗肿瘤活性相关分析 |
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| 引用本文： | 蒋洁君,尤奋强,马宏跃,周婧,张军峰,詹臻,唐于平,段金廒,丁安伟. 细胞连续亲和-HPLC测定8种蟾蜍甾烯与MGC-803细胞亲和量及抗肿瘤活性相关分析[J]. 中国中药杂志, 2011, 36(2): 205-208 |
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| 作者姓名： | 蒋洁君 尤奋强 马宏跃 周婧 张军峰 詹臻 唐于平 段金廒 丁安伟 |
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| 作者单位： | 1. 南京中医药大学,江苏省方剂研究重点实验室,江苏,南京,210046
2. 南京中医药大学,昆山附属医院,江苏,昆山,251300 |
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| 基金项目： | 国家自然科学青年基金项目(30901894);教育部新教师基金项目(20093237120013);霍英东教育基金会第十二届高等院校青年教师基金项目(121044);江苏省中医药局项目(LZ09017);南京中医药大学青年自然基金项目(09XZR21) |
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| 摘 要： | 目的:采有细胞连续亲和研究蟾蜍甾烯(Bu)与肿瘤细胞的生物亲和作用,分析其与抗肿瘤活性的相关性.方法:将蟾酥氯仿提取物与胃癌细胞MGC-803混合培养,高效液色谱测定上清液和细胞内8种Bu的含量,计算亲和率.结果:亲和后细胞上清液中Bu总减少率、细胞内游离Bu的含量百分率和两者差值(与细胞裂解物的亲和率)、抑制MGC-803活性(1/IC50)相关数r分别是0.82(P<0.05),-0.04,0.83(P<0.05).结论:8种蟾蜍甾烯与MGC-803发生不同程度亲和.其与肿瘤细胞裂解物的亲和率与抗肿瘤活性具有显著相关性.
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| 关 键 词： | 蟾蜍甾烯 MGC-803 细胞连续亲和 高效液相色谱 |
| 收稿时间： | 2010-03-26 |
Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities |
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| JIANG Jiejun,YOU Fenqiang,MA Hongyue,ZHONG Jing,ZHANG Junfeng,ZHAN Zhen,TANG Yuping,DUAN Jin''ao and DING Anwei. Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities[J]. China Journal of Chinese Materia Medica, 2011, 36(2): 205-208 |
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| Authors: | JIANG Jiejun YOU Fenqiang MA Hongyue ZHONG Jing ZHANG Junfeng ZHAN Zhen TANG Yuping DUAN Jin''ao DING Anwei |
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| Affiliation: | Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Affiliated Hospital, Nanjing University of Chinese Medicine, Kunshan 215300, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China |
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| Abstract: | Objective : To study the bioaffinity between 8 bufadienolides(Bu) and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu. Method : Mix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supernatant and cells using HPLC and calculate their affinity rate. Result : The coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r=0. 82 (P<0. 05),r=-0. 04 and r=0. 83(P<0. 05) respectively. Conclusion : Eight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities. |
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| Keywords: | bufadienolides MGC-803 cells cell affinity HPLC |
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