采用PCR-RFLP技术检测中国人HLA纯合细胞DQ基因多态性

本文采用PCR-RFLP方法对7株中国人HLA纯合细胞的DQA1、DQB1基因作基因分型,检出了这些细胞株的DQ基因类型,证实了中国人群存在着一种DRw12与DQA1*0601-DQB1*0301连锁的新单倍型。

ANALYSIS OF THE POLYMORPHIC DQ GENES OF CHINESE HLA HOMOZYGOTES WITH PCR-RFLP METHOD

The second exons of HLA-DQA1, DQB1 genes of Chinese homozygous cell lines were amplified with polymerase chain reaction (PCR), followed by digestion of the amplified DNA segments with allele-specific restriction endonucleases. The resulted patterns of restriction fragment length polymorphism (PCR-RFLP) in polyacrylamide gel eleetrophoresis were used for HLA-DQA1 and-DQB1 genotyping. With advantages such as timesaving,accuracy and omission of radioisotopes(to label oligonuclectide probes for hybridization), the technique has proved to be capable of genotyping Chinese homozygous cell lines (when compared with the PCR-RFLP patterns of reference cell lines). In addition, the results confirmed a new Chinese-specific haplotype, in which DRwl2 was associated with DQAl*0601-DQBl*0301.