|
|||||
|
|
| HPLC法测定复方沙棘籽油栓中蛇床子素的含量 | |
| 引用本文： | 尚飞,王亚侠,陈芳晓,钱春梅.HPLC法测定复方沙棘籽油栓中蛇床子素的含量[J].西北药学杂志,2006,21(4):147-148. |
| 作者姓名： | 尚飞 王亚侠 陈芳晓 钱春梅 |
| 作者单位： | 1. 西安交通大学药学系,陕西,西安,710061;西电集团医院药剂科,陕西,西安,710077 2. 西安铁路局,陕西,西安,710054 3. 西安交通大学药学系,陕西,西安,710061 |
| 摘 要： | 目的测定复方沙棘籽油栓中主要成分蛇床子素的含量。方法采用HPLC法,C8色谱柱;流动相为甲醇-水(75∶25);流速0.7 mL.min-1;检测波长320 nm;柱温30℃;进样量为10μL。结果蛇床子素含量与峰面积线性关系良好,回归方程为A×10-3=-7.950+7.384 X,r=0.999 5(n=5),平均回收率为98.3%,RSD值为0.71%。结论HPLC法测定复方沙棘籽油栓中蛇床子素的含量可行。 |
| 关 键 词： | 复方沙棘籽油栓 蛇床子素 HPLC法 |
| 文章编号： | 1004-2407（2006）04-0147-02 |
| 收稿时间： | 2005-10-17 |
| 修稿时间： | 2005年10月17 |
Determination of osthole in Compound Shajiziyou Suppositories by HPLC |
|
| SHANG Fei,WANG Ya-xia,CHEN Fang-xiao,QIAN Chun-mei.Determination of osthole in Compound Shajiziyou Suppositories by HPLC[J].Northwest Pharmaceutical Journal,2006,21(4):147-148. | |
| Authors: | SHANG Fei WANG Ya-xia CHEN Fang-xiao QIAN Chun-mei |
| Abstract: | Objective To determine the contents of osthole in Compound Shajiziyou Suppositories.Methods The HPLC conditions were as follows: C_(8)column,methanol-water(75∶25) as mobile phase,0.7 mL·min~(-1) as flow-rate and 320nm as detective wavelength;The column temperature was 30℃;The volume of injection was 10 μL.Results This method had a good linearity between osthole concentration and peak area.The linear equation was A×10~(-3)=-7.950+7.384X,r=0.9995(n=5).The average recovery was 98.3%,with RSD(0.71%).Conclusion This method can be used to determine osthole in Compound Shajiziyou suppositories. |
| Keywords: | Compound Shajiziyou Suppositories osthole HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |