丙型、乙型肝炎病毒在人原发性肝内胆管细胞癌中分布的免疫组化研究

应用原位分子杂交及免疫组化方法，使用地高辛标记ＨＣＶ５＇非编码区探针及抗ＨＣＶＮＳ３区Ｃ３３ｃ单克隆抗体检测３５例人原发性肝内胆管细胞癌，癌旁肝组织内ＨＣＶＲＮＡ及其ＮＳ３抗原，发现ＨＣＶＲＮＡ在肝内胆管细胞癌中的阳性率为８３％。ＨＣＶＲＮＡ定位于肝细胞胞浆中，个别病例在淋巴细胞、肝窦内皮细胞和枯否细胞中发现阳性信号。ＨＣＶＮＳ３区Ｃ３３ｃ抗原在肝内胆管细胞癌中的阳性率为８９％，阳性反应主要位于癌细胞胞浆内或肝细胞胞浆内，阳性细胞在癌组织中以灶性分布为主，在癌旁肝组织以弥漫分布为主。ＨＢ－ｘＡｇ在本组病例的阳性率为７７％。本文结果提示，在肝内胆管细胞癌的发生中，除ＨＢＶ以外，ＨＣＶ感染与其发生也有密切关系，是其致病因素之一。

IMMUNOHISTOCHEMICAL STUDY OF HCV,HBV IN HUMAN PRIMARY INTRAHEPATIC CHOLANGIOCARCINOMA

HCV RNA and its NS3 region C33c antigen were detected by in situ hybridization and immunohis tochemical methods in 35 cases of primary intrahepatic cholangiocarcinoma(PIC ) tissues. The positive rate of HCV RNA in PIC was,83%and the HCV RNA was localized in the cytoplasm and/or nuclei of cancerous cell and hepatocytes. In one case,HCV RNA was also detected in nuclei of lymphocyte and sinusoidal cell/or Kupffer's cells. The positive rate of HBV NS3 region C33c antigen was 89%in PIC,positive reaction was found in the cyto- plasm of cancerous cell and hepatocytes,The positivc cells were distributed locally and diffusely. HBxAg was also detected in 77%of the patients studied.Our resuIts suggested that HCV infection close correlation with the de- velopment of PIC except HBV infection. HCV may play an important rloe in the carcinogenesis of PIC.