| 转染wt-53基因对SHG44细胞恶性表型的影 |
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| 引用本文： | 宋祖军,章翔. 转染wt-53基因对SHG44细胞恶性表型的影[J]. 医学争鸣, 2000, 21(8): 952-953 |
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| 作者姓名： | 宋祖军 章翔 |
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| 作者单位： | 1. 第四军医大学西京医院:急诊科,陕西,西安,710033
2. 第四军医大学西京医院:全军神经外科研究所,陕西,西安,710033 |
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| 摘 要： | 目的：转染ｗｔ－ｐ５３基因对ＳＨＧ４４细胞恶性表型的影响。方法脂质体介导法，将ｗｔ－ｐ５３基因转导入ＳＨＧ４４人胶质瘤细胞，斑点霜交证实转染成功；观察阳性转染细胞在体外常规培养中生长速率，细胞培增时间；平板集落形成率，结果转染ｗｔ－ｐ５３的细胞生长速度明显减慢，倍增时间延长（Ｐ〈０．０１）；细胞集落形成数明显减少（Ｐ〈０．０１），结论外源性野生型Ｐ５３基因对ＳＨＧ４４细胞的恶性表型有抑制作用。
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| 关 键 词： | p53基因 恶性表型 基因转染 SHG44细胞 肿瘤 |
| 修稿时间： | : |
The effect of wild-type p53 gene transfection on malignant phenotype of SHG44 cell line |
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| SONG Zu-Jun,ZHANG Xiang. The effect of wild-type p53 gene transfection on malignant phenotype of SHG44 cell line[J]. Negative, 2000, 21(8): 952-953 |
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| Authors: | SONG Zu-Jun ZHANG Xiang |
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| Abstract: | AIM To study the effect of wild type p53 gene transfection on malignant phenotype of SHG 44 cell line. METHODS Wild type p53 gene were transfected into human glioma cell line SHG 44 cells with liposome. The growth rate, cell double time and colony formation rate of transfected cells were observed. RESULTS The growth rate of wild type p53 gene transfected cells declined obviously, the double time extended ( P <0.01) and the number of colony formation significantly decreased ( P <0.01). CONCLUSION Exogenous wild type p53 gene could inhibit the malignant phenotype of SHG 44 cell line. |
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| Keywords: | p53 gene malignant phenotype gene transfection SHG 44 cell |
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