皮肤鳞状细胞癌中细胞外基质的分布形式与意义

目的 观察细胞外基质中纤连蛋白、层粘连蛋白在皮肤鳞状细胞癌(鳞癌)中的分布形式,探讨其与癌生物学行为的关系.方法 用纤连蛋白、层粘连蛋白、增殖细胞核抗原、p53抗体对50例皮肤鳞癌作免疫组化染色.结果 纤连蛋白、层粘连蛋白在皮肤鳞癌中显示3种分布形式,不连续巢周型、碎片型、血管间质型,这3种分布形式与癌生长、分化、增殖密切相关,3例分化好的膨胀巢状生长,在癌巢周有连续线状纤连蛋白、层粘连蛋白分布.结论 皮肤鳞癌中纤连蛋白、层粘连蛋白明显减少,浸润性癌中不一定均有基膜缺乏,癌浸润后可能需经过灶性组织分化,再进一步浸润与转移,纤连蛋白、层粘连蛋白的分布形式反应了癌的恶性程度.

Distribution of Extracellular Matrix in Squamous Cell Carcinoma of Skin and Its Biological Significance

Objective To find out the distribution of extracellular matrix (ECM) in squamous cell carcinoma (SSC) and explore its biological significance. Methods Fifty cases of SSC were studied by immunohistochemistry with antibodies to laminin (LN), fibronectin (FN), PCNA, and p53. Results Three types of distribution patterns were found. i.e., discontinuous peri carcinoma nest type, fragmentary type, and vascular stroma type. The three types were correlated closely with the growth, differentiation and proliferation of tumor. Basement membrane was present in 3 cases with large invasive tumor nests, shown by continuous linear distribution of FN and LN. Conclusion The expressions of FN and LN decrease markedly, but the absence of basement membrane does not necessarily define invasive SSC, and the correlation of the presence of basement membrane and histologic pattern of invasive tumor suggests that SSC is capable of undergoing focal differentiation before invasion and metastasis have occurred. The distribution of FN and LN might represent the malignant degree of SSC.