Characterization of An Avian Influenza Virus of Subtype H7N2 Isolated from Chickens in Northern China |
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Authors: | Yanbing Li Chengjun Li Liling Liu Hongwei Wang Chuanbin Wang Guobing Tian Robert G Webster Kangzhen Yu Hualan Chen |
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Institution: | (1) Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People’s Republic of China;(2) Veterinary Diagnosis Center of the Ministry of Agriculture, China Agriculture University, 2 Yuanminyuan West Road, Beijing, 100094, People’s Republic of China;(3) Division of Virology, Department of Infectious Diseases, St. Jude Children’s Research Hospital, 332 N. Lauderdale, Memphis, TN 38105, USA;(4) National Animal Husbandry and Veterinary Service of the Ministry of Agriculture, 20 Maizidian Street, Beijing, 100026, People’s Republic of China |
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Abstract: | An H7N2 avian influenza virus was isolated from chickens during routine surveillance in northern China in 2002. To understand
the origin of this virus, we completely sequenced its genome. The PB1, PA, HA, and M genes of this virus were highly homologous
with those of the wild bird virus A/Africa starling/Eng-Q/983/79 (H7N1). The NP and NS genes were closely related to those
of two other wild bird viruses isolated 30 years ago. The closest relatives of the PB2 and NA genes of the virus were those
of the A/swine/Germany/2/81 (H1NI) and A/Leningrad/134/57 (H2N2), respectively. Animal inoculation tests showed that the virus
cannot replicate efficiently in chickens. However, after intranasal inoculation, the virus induced 20% weight loss and replicated
well in the lungs of mice. The virus was also recovered from the hearts and brains of the mice. These results suggest that
the influenza virus isolated in chickens in northern China in 2002 originated in wild birds and may pose a threat for both
avian species and mammalian hosts. |
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Keywords: | Avian influenza virus H7N2 subtype Genetic analysis Pathotyping Chicken Mouse |
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