高效液相色谱法测定沉香曲中羌活醇、异欧前胡素、木香烃内酯和去氢木香内酯的含量

目的建立HPLC法测定沉香曲中羌活醇、异欧前胡素、木香烃内酯和去氢木香内酯的含量。方法色谱柱为Hypersil C18柱(4.6 mm×250 mm,5μm);流速:0.9 mL·min-1;检测羌活醇和异欧前胡素的流动相为乙腈-0.5%磷酸溶液(67:33),检测波长:312 nm;检测木香烃内酯和去氢木香内酯的流动相为乙腈-甲醇-1%冰醋酸溶液(45:24:31),检测波长:225 nm。结果羌活醇和异欧前胡素进样量分别在0.018 6⁰.372 0μg(r=0.999 2)、0.011 20.372 0μg(r=0.999 2)、0.011 2⁰.224 0μg(r=0.999 1)与峰面积呈良好的线性关系,平均加样回收率分别为96.6%、98.0%,RSD分别为1.6%、1.2%(n=6);木香烃内酯和去氢木香内酯进样量分别在0.041 40.224 0μg(r=0.999 1)与峰面积呈良好的线性关系,平均加样回收率分别为96.6%、98.0%,RSD分别为1.6%、1.2%(n=6);木香烃内酯和去氢木香内酯进样量分别在0.041 4⁰.828 0μg(r=0.999 5)、0.010 80.828 0μg(r=0.999 5)、0.010 8⁰.216 0μg(r=0.999 7)与峰面积呈良好的线性关系,平均加样回收率分别为98.0%、96.2%,RSD分别为1.4%、1.1%(n=6)。结论该方法测定结果准确性强、灵敏度高、重复性好。

Determination of notopterol,isoimperatorin, costunolide and dehydrocostuslactone in Chenxiangqu by HPLC

Objective To develop an HPLC method for the content determination of notopterol, isoimperatorin, costunolide and dehydrocostuslactone in Chenxiangqu. Methods An Hypersil C18 column was used as the chromatographic column, and the flow rate was 0.9 mL · min- 1. Notopterol and isoimperatorin： the mobile phase consisted of acetonitrile-0.5% phosphoric acid solution（67 ：33）. The UV detection wavelength was 312 nm. Costunolide and dehydrocostuslactone： the mobile phase consisted of acetonitrile-methanol-1% acetic acid solution（45 ：24 ：31）. The UV detection wavelength was 225 nm. Results There was a good linearity between the concentration of notopterol, isoimperatorin and peak area value when the concentrations of notopterol and isoimperatorin were at 0.018 6- 0.372 0 μg（r = 0.999 2） and 0.011 2- 0.224 0 μg（r = 0.999 1）. The average recovery was 96.6%（RSD = 1.6%） and 98.0%（RSD = 1.2%）. There was a good linearity between the concentration of costunolide, dehydrocostuslactone and peak area value when the concentrations of costunolide and dehydrocostuslactone were at 0.041 4- 0.828 0 μg（r = 0.999 5） and 0.010 8- 0.216 0 μg（r = 0.999 7）. The average recovery was 98.0%（RSD = 1.4%） and 96.2%（RSD = 1.1%）. Conclusion The method is accurate, sensitive, reproducible and can be used in the determination of notopterol, isoimperatorin, costunolide and dehydrocostuslactone in Chenxiangqu.