Grb2在胰腺癌中的表达及其意义

目的：探讨生长因子受体结合蛋白2（Grb2）在胰腺癌中的表达及其意义。 方法：分别采用real-time PCR和Western blot方法检测6种胰腺癌细胞株中Grb2基因和蛋白表达水平；采用组织芯片检测80例胰腺癌组织及癌旁胰腺组织中Grb2的表达情况，并分析Grb2表达状态与胰腺癌患者临床病理特征的关系。 结果：Grb2的mRNA与蛋白在高侵袭力、低分化细胞株PANC-1、MIA-PaCa-2、AsPC-1中呈相对高表达，而在低侵袭力、高分化细胞株BxPC-3、SW1990、capan-2中呈相对低表达；Grb2在胰腺癌组织中均有表达，而在癌旁胰腺组织中不表达或低表达，统计分析显示，Grb2的高表达与肿瘤低分化以及淋巴结或者远处转移有关（均P<0.05）。 结论：Grb2的表达与胰腺癌的分化程度和侵袭转移力密切相关，高表达者肿瘤恶性程度高，预后差。

Grb2 expression in pancreatic carcinoma and its significance

Objective: To investigate the Grb2 expression in pancreatic carcinoma and its clinical significance. Methods: The Grb2 gene and protein expressions in 6 types of pancreatic cancer cell lines were detected by real-time PCR and Western blot, respectively. The Grb2 protein expressions in pancreatic cancer tissues and their adjacent normal tissues from 80 cases were determined by tissue microarray technique, and the relations of Grb2 expression status with clinicopathologic profiles of the pancreatic cancer patients were analyzed. Results: Both the Grb2 mRNA and protein showed relatively high expression level in the highly invasive and poorly differentiated pancreatic cell lines PANC-1, MIA-PaCa-2 and AsPC-1, but presented relatively low expression level in the less invasive and well differentiated pancreatic cell lines BxPC-3, SW1990 and capan-2. The Grb2 expression was detected in all tested pancreatic cancer tissues, but no or weak Grb2 expression was found in their adjacent normal pancreatic tissues. Statistical analysis showed that high Grb2 expression was associated with poor differentiation of the tumor and lymph node or distant metastasis (both P<0.05). Conclusion: Grb2 expression is closely related to the degree of differentiation and ability of invasion and metastasis of pancreatic cancer, and high Grb2 expression may predict highly malignant tumor grades and poor outcome.