脑缺血再灌注肾脏损伤老龄大鼠的单胺类神经递质和神经肽的变化意义

目的：从多巴胺(DA)、去甲肾上腺素(NE)、肾上腺素(E)、内皮素(ET)和降钙素基因相关肽(CGRP)的变化方面揭示老龄大鼠脑缺血再灌注肾脏损伤机制。方法：青年(5月龄)和老龄(20月龄以上)大鼠均分为模型组和正常对照组，观察大鼠全脑缺血30min再灌注60min后肾脏组织形态和肌酐(Cr)、尿素氮(Bun)、DA、NE、E、ET、CGRP含量的变化。结果青年和老龄模型组大鼠肾脏组织形态和功能均出现明显的病理改变，交感-肾上腺系统兴奋性增强，老龄模型组较青年模型组严重。青年对照组血浆中CGRP高于青年模型组和老龄对照组。老龄模型组血中ET高于老龄对照组和青年模型组。结论：大鼠脑缺血再灌注肾脏损伤与交感-肾上腺系统兴奋性增强以及CGRP与ET的平衡失调有关，由于增龄的变化使老龄大鼠脑缺血再灌注肾脏损伤程度和这些病理变化尤为明显并具有一定特点。

Significance of Monoamine Neurotransmitters and Neuropeptide Content Changes in the Senile Rats with Renal lnjury after Brain lschemia Reperfusion

Objective To study the mechanism of renal injury after brain ischemia reperfusion in aged rats with the changes of dopamine (DA), noradrenalin(NE), epinephrine(E), endothelin(ET), calcitonin gene related peptide(CGRP).Methods Young(5 months) and aged (20 months or more) rats were divided into young control group(YCG), young model group(YMG), aged control group(ACG), and aged model group(AMG).The following items were observed in rats with 60 min reperfusion after 30 min brain ischemia involved:the pathology of kidney, and the contents of creatinine(Cr), urea nitrogen(Bun), DA, NE, E, ET, and CGRP.Results The distinct pathological and functional injury in the kidney, the excitability of sympatho adrenal system were found in the YMG, and AMG groups. The CGRP level in the YCG was higher than that in the YMG and the ACG. The plasma ET content in the AMG was higher than that in the ACG and the YMG.Conclusions The kidney injury after brain ischemia reperfusion was concerned with the enhanced excitability of sympathetic adrenal system and the dysequilibrium between CGRP and ET. The degree of this injury and pathological change were distinctive and characteristic because of the aging changes.