PLLA-PTX微粒的缓释及对人卵巢癌SKOV3细胞的抑瘤作用

目的 证明聚左旋乳酸-紫杉醇(PLLA-PTX)微粒影响SKOV3细胞增殖和凋亡的有效性;探讨生物降级高分子材料聚左旋乳酸(PLLA)对所担载抗肿瘤药物紫杉醇(PTX)的缓释作用.方法 实验分为PLLA-PTX微粒组、PTX组和空白对照组.MTT比色法检测细胞的增殖情况;倒置显微镜观察细胞形态学变化; FCM技术进行细胞凋亡及周期分析;TUNEL 检测、定位凋亡细胞.结果 PLLA-PTX微粒能有效的抑制SKOV3细胞的增殖,具剂量和时间依赖性.其抑瘤作用明显优于裸露的PTX,随时间的延长,优势不断增大.PLLA-PTX微粒的最佳实验浓度为0.05 μmol/L.倒置显微镜下,PLLA-PTX微粒组和PTX组培养液中漂浮大量体积变圆的细胞和颗粒状无定形碎片,随时间的延长,PLLA-PTX微粒组更明显.PLLA-PTX微粒组和PTX组肿瘤细胞凋亡率均高于对照组(P<0.05).随时间的延长,PLLA-PTX微粒提高细胞凋亡率、增加G2/M期细胞比例的优势大于PTX组.TUNEL可以准确定位PLLA-PTX微粒作用后正在凋亡的细胞,同时出现了两种独特细胞:病理性核分裂象细胞和多微核细胞. 结论 PLLA-PTX微粒具有较强的细胞毒作用及明显的缓释作用,可以维持较长时间的有效浓度,持续抑制SKOV3细胞的增殖.

Controlled Release of Paclitaxel from Microparticles Containing PLLA and Its Anti-tumor Activity on Human Ovarian Carcinoma Cell Line SKOV3

Objective To prove the PLLA-PTX' efficacy on growth,apoptosis of ovarian cancer cell line SKOV3,and to study the controlled release roles of PLLA for PTX.Methods Cultured cells of human ovarian Carcinoma cell Line SKOV3 were treated with PLLA-PTX microparticles and PTX only separately,untreated cells as the control.The proliferation of SKOV3 cells were determined by MTT assay with the morphologic change observed under inverted phase contrast microscope,the apoptosis of cell were demonstrated by FCM and in s...